Determination of Polysaccharide and Their Monosaccharide Composition from Polygonatum and Odoratum by CZE
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Determination of Polysaccharide and Their Monosaccharide Composition from Polygonatum and Odoratum by CZE
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 6, Pages: 54-59(2011)
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Published:2011
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GUO Huai-zhong, CHEN Chun-ying, ZHAO Huan-rong. Determination of Polysaccharide and Their Monosaccharide Composition from Polygonatum and Odoratum by CZE[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 54-59.
DOI:
GUO Huai-zhong, CHEN Chun-ying, ZHAO Huan-rong. Determination of Polysaccharide and Their Monosaccharide Composition from Polygonatum and Odoratum by CZE[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 54-59.DOI:
Determination of Polysaccharide and Their Monosaccharide Composition from Polygonatum and Odoratum by CZE
Objective: The method of determining polysaccharides and their monosaccharides composition by CZE was established to provide a reference for discussing the effective substance of Polygonatum and Odoratum. Method: The degradation conditions of Polygonatum and Odoratum polysaccharides were optimized by orthogonal tests. The degradative monosaccharides
which were derived with 1-phenyl-3-methyl-5-pyrazolone (PMP)
were separated and determined by CZE
and the results were compared with those which were determined by phenol-sulfuric acid method. Result: The Polygonatum and Odoratum monosaccharides all included xylose
arabinose
glucose
rhamnose
mannose
galactose
glucuronic acid and galacturonic acid. However
the ratio was significant different. Conclusion : The analysis of Polygonatum and Odoratum polysaccharides and the composition of monosaccharide by CZE are accurate and reliable with satisfactory results. The similarities and differences of the effectiveness were evidenced by their monosaccharide composition in some degree
and the results suggested that their clinical application could not be confused.
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Related Institution
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