Technical Study and Comparison of Total Flavonoids Extracted in  Leaves of Different Picked Time by Ultrasonography

WANG Han-qing; WANG Wen-ping; YAN Jin-jin; SUI Hong

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Technical Study and Comparison of Total Flavonoids Extracted in Leaves of Different Picked Time by Ultrasonography

更新时间：2024-01-04

- Technical Study and Comparison of Total Flavonoids Extracted in Leaves of Different Picked Time by Ultrasonography
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 8, Pages: 44-47(2011)
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- Published：2011
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WANG Han-qing, WANG Wen-ping, YAN Jin-jin, et al. Technical Study and Comparison of Total Flavonoids Extracted in Leaves of Different Picked Time by Ultrasonography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 44-47.
DOI：

WANG Han-qing, WANG Wen-ping, YAN Jin-jin, et al. Technical Study and Comparison of Total Flavonoids Extracted in Leaves of Different Picked Time by Ultrasonography[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 44-47. DOI：

摘要

目的: 优选超声辅助提取宁夏枸杞叶总黄酮的提取工艺并测定其不同采收期总黄酮含量。方法: 分别研究乙醇浓度

乙醇用量

超声时间

超声温度4个单因素对提取效果的影响

并设计正交试验

所得结果采用方差分析。利用优选出的最佳超声提取工艺测定比较不同采收期枸杞叶中的总黄酮含量。结果: 确定了最佳单因素水平

通过正交试验优选出超声辅助提取枸杞叶总黄酮的最佳工艺条件为乙醇体积分数65%、乙醇用量1 ∶60、超声提取时间35 min、超声温度70 ℃。结论: 通过比较不同采收时期枸杞叶总黄酮的含量

结果为5月中旬含量最高。

Abstract

Objective: To optimally select the extraction technology of total flavonoids of Lycium barbarum leaves by ultrasonography and to determine the content of total flavonoids of L.barbarum leaves of different picked time. Method: The effects of four single-factors (including ethanol concentration

ethanol consumption

ultrasonic extraction time and temperature)on the extraction of total flavonoids of L. barbarum leaves have been studied.And the results of the orthogonal experiment were analyzed by variance analysis. Use the best extraction technology to determine the contents of total flavonoids in leaves of L.barbarum at different picked times. Result: The best single factor was determined and the best extraction of total flavonoids of L. barbarum leaves by ultrasonography with orthogonal experiment was optimized. The best extraction technology were as follows: using 65% ethanol as extractor with the ratio of material to liquid at 1 ∶60 and extraction for 35 min in 70 ℃. Conclusion: By comparing the contents of total flavonoids in leaves of L. barbarum at different picked time

the contents of total flavonoids in leaves were decreased with the leaves growing. It is conclude that the best picked time of L. barbarum is the middle of May.

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Related Author

WANG Han-qing

ZHANG Xia

LU Rui

WANG Han-qing1

ZHANG Xia1

WANG Wen-ping1

LU Rui1

SUN Hong

Related Institution

Ningxia Research Institute of Medicine & Pharmacy,Ningxia Medical University

Ningxia Research Center of Modern Hui Medicine Engineering and Technology

College of Pharmaceutics,Ningxia Medical University

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

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