Comparison of Protein Extraction Methods of Trichosanthis Fructus

SONG Yang-mei; LI Feng; ZHOU Li-man

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Comparison of Protein Extraction Methods of Trichosanthis Fructus

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- Comparison of Protein Extraction Methods of Trichosanthis Fructus
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 4, Pages: 95-97(2011)
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- Published：2011
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SONG Yang-mei, LI Feng, ZHOU Li-man. Comparison of Protein Extraction Methods of Trichosanthis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 95-97.
DOI：

SONG Yang-mei, LI Feng, ZHOU Li-man. Comparison of Protein Extraction Methods of Trichosanthis Fructus[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 95-97. DOI：

摘要

目的 :建立适合于瓜蒌种子的蛋白质提取方法。方法 :比较水溶液直接提取法(3种)、三氯乙酸(TCA)-丙酮法、三羟甲基氨基甲烷(Tris)-丙酮法、蛋白质提取液提取法等6种蛋白质提取方法所获瓜蒌种子总蛋白含量、聚丙烯酰胺凝胶电泳的谱带等方面的差异。结果 :蛋白质提取液提取法、TCA-丙酮法提取蛋白含量较高

但杂质多

电泳谱带严重展宽而且明显拖尾;Tris-丙酮法提出的蛋白质种类缺失严重;直接提取法提取蛋白含量少

但杂质少

电泳谱带清晰

离心2次谱带更加清晰。结论 :水溶液直接提取法最适合用于聚丙烯酰胺凝胶电泳

蛋白质提取液提取法提出蛋白质含量最多。

Abstract

Objective: To determine the protein extraction method of Fructus Trichosanthis. Method :The protein yield

protein purity and polyacrylamide gel electrophoresis(PAGE) patterns were determined and analyzed. Result: More proteins were extracted by the method of protein extracted liquid and trichloroacetic acid(TCA)-acetone

but their bands were not clear and the resolution was low because of more impurities extracted.It waseasy to produce high-abundance proteins and interfered in the electrophoresis resolution.Protein yield and band numbers by Tris-base/acetone were the lowest.Besides

the protein content by commonly used direct extraction was low

but with less impurity

clearer bands and highest resolution. Conclusion: Direct extraction is the most suitable method for PAGE and more protein can be extracted by protein extracted liquid extraction method.

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Related Author

SONG Yang-mei

LI Feng

ZHOU Li-man

Meng LAN

Xue-feng BIAN

Jing-wei LYU

Hui ZHANG

Hui YAO

Related Institution

College of Pharmacy,Shandong University of Traditional Chinese Medicime

Jilin Ginseng Academy in Changchun University of Chinese Medicine

Jilin Xinshui Science and Technology Development Co. Ltd.

Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

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