Determination of -Terthienyl in Radix Echinopsis from Different Places by RP-HPLC

LI Xi-feng; YU Yun-hui; HU Chun-yue; HU Li-xin; QIU Tian-bao; GAO Wei-li

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Determination of -Terthienyl in Radix Echinopsis from Different Places by RP-HPLC

更新时间：2024-01-04

- Determination of -Terthienyl in Radix Echinopsis from Different Places by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 6, Pages: 60-62(2011)
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- Published：2011
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LI Xi-feng, YU Yun-hui, HU Chun-yue, et al. Determination of -Terthienyl in Radix Echinopsis from Different Places by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 60-62.
DOI：

LI Xi-feng, YU Yun-hui, HU Chun-yue, et al. Determination of -Terthienyl in Radix Echinopsis from Different Places by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 60-62. DOI：

摘要

目的: 采用RP-HPLC法测定不同产地禹州漏芦中α-三联噻吩含量。方法: 色谱柱为Hypersil ODS2C18 (4.6 mm×250 mm

5 μm)

流动相甲醇-0.1%醋酸溶液(85 ∶15)

流速1.0 mL ·min-1

柱温25 ℃

检测波长352 nm。结果: α-三联噻吩在0.491 6~6.145 0 μg峰面积与进样量呈良好线性关系(r=0.999 9)

平均加样回收率为98.83%。结论: 该法简便、快速、准确、专属性强、重现性好

不同产地禹州漏芦中α-三联噻吩含量均符合《中国药典》(2010年版)不得少于0.2%的规定。

Abstract

Objective: Determination of α-terthienyl in Radix Echinopsis from different places by using RP-HPLC. Method: The separation was carried out at 25 ℃ on a Hypersil ODS2 C18 column (4.6 mm×250 mm

5μm) with MeOH 0.1% acetate solution (85 ∶15) as mobile phase. The detective wavelength was at 352 nm with the flow rate of 1.0 mL ·min-1. Result: The α-terthienyl was linear within the concentration range of 0.4916-6.1450 μg (r=0.999 9). The average recovery was 98.83%. Conclusion: This method is simple and practical with strong specificity and good reproducibility.The content of α-terthienyl is up to the regulation of no less than 0.2% in Chinese Pharmacopoeia (Edition 2010) from different places.

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Related Author

LI Xi-feng

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Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

Sunflower Pharmaceutical Group（Xiangyang） Longzhong Co. Ltd.，Xiangyang

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences，Beijng

Shanxi University of Chinese Medicine

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