Objective: HPLC fingerprints of the hemostatic fraction of Rehmannia glutinosa were established. Method: Akasil C18 (4.6 mm×250 mm

5 μm) column was used and the acetonitrile-water was chosen as the mobile phase in a gradient mode.The column temperature was at 30 ℃ and the detection wavelength was at 210 nm.The detection time was 60 min

and the flow rate was 1.0 mL ·min-1. Result: Fifteen characteristic peaks were indicated in HPLC fingerprints.The relative retention time is as of the common peaks were also determined. Conclusion: The method established is simple and accurate with a good reproducibility and will provide a reference standard for the quality control of R.glutinosa .