Protective Effect of  on Photoaging Induced by Ultraviolet Radiation in ESF-1 Cell

XU Yan-ming; ZHANG Ning; JING Li-wei; CHEN Qiao-yun; QI Yong-hua; LI Jian-min

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Protective Effect of on Photoaging Induced by Ultraviolet Radiation in ESF-1 Cell

更新时间：2024-01-04

- Protective Effect of on Photoaging Induced by Ultraviolet Radiation in ESF-1 Cell
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 7, Pages: 120-123(2011)
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- Published：2011
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XU Yan-ming, ZHANG Ning, JING Li-wei, et al. Protective Effect of on Photoaging Induced by Ultraviolet Radiation in ESF-1 Cell[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 120-123.
DOI：

XU Yan-ming, ZHANG Ning, JING Li-wei, et al. Protective Effect of on Photoaging Induced by Ultraviolet Radiation in ESF-1 Cell[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 120-123. DOI：

摘要

目的: 研究杜仲抗紫外线致ESF-1细胞光老化作用

并初步探讨其作用机制。方法: 杜仲70%乙醇提取物上D101大孔树脂制备不同浓度乙醇梯度洗脱部位

采用40 J ·cm-2的UVA或70 mJ ·cm-2的UVB照射ESF-1细胞后

立即加入不同浓度的杜仲50%乙醇洗脱部位

24 h后MTT法测定细胞活性

试剂盒法测定细胞培养液中乳酸脱氢酶(LDH)、超氧化歧化酶(SOD)活力及丙二醛(MDA)含量。结果: 杜仲50%乙醇大孔树脂洗脱部位250

500 mg ·L-1的给药浓度能使UVA诱导的光老化细胞活性明显增强(P<0.05)

细胞培养液中LDH活力明显降低(P<0.05)

其中250 mg ·L-1的给药浓度能使细胞培养液中SOD活力明显升高(P<0.05)

MDA含量明显降低(P<0.05);125

250

500 mg ·L-1的给药浓度能使UVB诱导的光老化细胞活性明显增强

其中125 mg ·L-1的给药浓度能使细胞培养液中LDH活力明显降低(P<0.05)

SOD活力明显升高(P<0.05)

MDA含量明显降低(P<0.05)。结论: 杜仲50%乙醇大孔树脂洗脱部位对UVA和UVB致ESF-1细胞光老化具有良好的保护作用

推测其机制为通过提高SOD活力、加速氧自由基的清除和减少氧自由基的产生

使细胞的脂质过氧化损伤程度降低;同时

可能通过抗氧化作用

维持了细胞膜结构和功能的完整性

使LDH的漏出减少。

Abstract

Objective: To study the protective effect of Eucommia ulmoides agains UV irradiation-induced damage in ESF-1 cells and its mechanism. Method: E. ulmoides was extracted by 70 ethanol

then the extract was eluted with gradient of ethanol by macroporous resin

ESF-1 cells were immediately treated with different concentrations of 50% ethanol eluate after UVA irradiation (40 J ·cm-2) or UVB irradiation (70 mJ ·cm-2). Twenty-four hours later

the proliferation of cells was detected by MTT

SOD

LDH and MDA content were assayed by colrorimetry and TBA methods respectively. Result: Compared with the UVA group

the concentration of 125

250 mg ·L-1 could increase the proliferation(P<0.05) and reduce the activity of LDH (P<0.05). The concentration of 250 mg ·L-1 could increase the activitiy of SOD (P<0.05) and decreased the content of MDA (P<0.05). Compared with the UVB group

the concentration of 125

250 and 500 mg ·L-1could increase the proliferation (P<0.05). The concentration of 125 mg ·L-1could increase the activity of SOD (P<0.05) and reduce the activity of LDH (P<0.05) and decreased the content of MDA (P<0.05). Conclusion: The ethanol eluate of 50% can inhibit UV irradiation-induced damage in ESF-1 cells. Its mechanism is due to its abilities of increasing SOD

scavenging oxygen free radicals

inhibiting lipid peroxidation

and protecting membrane structure.

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