Objective: To investigate the effect of Siwu Decoction and its drug pairs on proliferation of rat ovary granulosa cell

and to probe the effect of combination of drug pairs. Method: Siwu Decoction and its drug pairs were added into the cultured rat ovary granulosa cells in vitro at the concentration of 2

20

200 mg ·L-1

and proliferation of the cells was measured by MTT. Result: Under the treatment of Siwu Decoction

the proliferation of granulosa cells was significant promoted by 36.73% in compare with the control group. The same effect was also found after treating with 5 drug pairs

and the order of promoting proliferation effects was: Angelicae Sinensis Radix-Chuanxiong Rhizoma (66.86%)>Angelicae Sinensis Radix-Paeoniae Radix Alba(58.52%)>Paeoniae Radix Alba-Chuanxiong Rhizoma(47.03%)>Paeoniae Radix Alba-Rehmanniae Radix Praeparata(41.43%)>Angelicae Sinensis Radix-Rehmanniae Radix Praeparata(23.67%). Water preparation (1 ∶2)

ethanol preparation (1 ∶1) and ethanol-water preparation (1 ∶1.5) showed the strongest activity in each group extracted by different method. Conclusion: Siwu Decoction and its drug pairs showed significant promotion effects on rat ovary granulosa cell proliferation. Different pairing ratios of Angelicae Sinensis Radix to Chuanxiong Rhizoma and different extraction procedure contribute to different effects on the proliferation of rat ovary granulosa cell in vitro.