Studies on Purification Technology of Total Fumaric Alkaloids
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Studies on Purification Technology of Total Fumaric Alkaloids
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 7, Pages: 40-43(2011)
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Published:2011
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SUN Jing, ZHANG Ming, ZHANG Xiao-fei. Studies on Purification Technology of Total Fumaric Alkaloids[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 40-43.
DOI:
SUN Jing, ZHANG Ming, ZHANG Xiao-fei. Studies on Purification Technology of Total Fumaric Alkaloids[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 40-43.DOI:
Studies on Purification Technology of Total Fumaric Alkaloids
Objective: To study the method of enrichment of fumaric alkaloids by using cation resin. Method: Ultraviolet spectrophotometry was used to determine total fumaric alkaloid with exchange capacity as index. Result: The optimum purification condition was as follows: herbs: concentrate solution was about 1 ∶5
herbs: cation resin was about 1 ∶1; flow rate was 2 BV ·h-1
diameter:high of cation resin column was about 1 ∶10; 5 BV of purified water eluted resin column to removed impurities
flow rate was 2 BV ·h-1
4 BV of 70% ethanol as eluent consisted of 5% ammonia
2 BV ·h-1 flow rate washing resin column. Conclusion: The optimum condition was stable and can be used for purification of alkaloids.
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