Distribution of Isorhynchophyline in Liver and Kidney of SHR Rats before and after Compatiblity of Unicariae Ramulus Cum Uncis with Gastrodiae Rhizoma
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Distribution of Isorhynchophyline in Liver and Kidney of SHR Rats before and after Compatiblity of Unicariae Ramulus Cum Uncis with Gastrodiae Rhizoma
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 7, Pages: 220-223(2011)
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Published:2011
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ZHANG Wei-guo, LI Xiao-qian, WANG Xing, et al. Distribution of Isorhynchophyline in Liver and Kidney of SHR Rats before and after Compatiblity of Unicariae Ramulus Cum Uncis with Gastrodiae Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 220-223.
DOI:
ZHANG Wei-guo, LI Xiao-qian, WANG Xing, et al. Distribution of Isorhynchophyline in Liver and Kidney of SHR Rats before and after Compatiblity of Unicariae Ramulus Cum Uncis with Gastrodiae Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 220-223.DOI:
Distribution of Isorhynchophyline in Liver and Kidney of SHR Rats before and after Compatiblity of Unicariae Ramulus Cum Uncis with Gastrodiae Rhizoma
Objective: To study the distribution of isorhynchophyline in liver and kidney tissues of SHR rats before and after compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Unics Le
to investigate the impact of channel tropism before and after combination. Method: Uncariae Ramulus Cum Uncis decoction group was administrated Uncariae Ramulus Cum Unics
Tianma Gouteng decoction group was administrated with decoction of compatible Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis at a dose of 1.172 g ·kg-1. A blank group was received normal saline. Then using HPLC to determinate the isorhynchophyline in liver and kidney tissues of SHR rats before and after the compatibility of Gastrodiae Rhizoma with Unicariae Ramulus Cum Uncis. Analytical column was WatersTM(4.6 mm×250 mm
RP 18.5 μm). The mobile phase consisted of methanol-water (70 ∶30)
pH 8.0
rate was 10.8 mL · min-1. The detector wave length was set at 254 nm
the column temperature was 30 ℃. Result: Isorhynchophyline in liver and kidney tissues was linear in the range of 0.08-16.0 mg ·L-1. There existed significant (P<0.05) differences in liver and kidney tissues before and after the compatibility. Conclution :The compatibility of Chinese medicine has close correlation with channel tropism.
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Related Institution
School of Life Science and Engineering,Southwest Jiaotong University
Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine, Henan University of Chinese Medicine
College of Agronomy, Henan Agricultural University
Beijing University of Chinese Medicine
School of Traditional Chinese Medicine(TCM),Shanghai University of TCM