Objective: To screen the active fraction of compound Sanhuang capsules for inhibiting angiogenesis in tumor. Method: Experimental study of tumor angiogenesis in vivo was carried out with model of chorioallantoic membrane. Result: ①Mixed and suspended the ultrafine powder of compound Sanhuang capsules

which were irradiated by Co60 at the seventh day

with ethanol in different concentrations.Then added it into gelatin sponge

the diameter was 5 mm and under the ultraviolet radiation.Two days later

around the gelatin sponge appeared round and radicalized capillary vessels.the number of the blood vessel was fewer than that in the normal saline control group (P<0.05); there ware no significant differences between Taxol group and normal saline control group.②Extracted the irradiated ultrafine powder of compound Sanhuang capsules with ethyl acetate before

next used pH gradient method to extract the different positions

and then each part tested with chorioallantoic membrane(CAM) model.At last the vessel around the gelatin sponge decreased

compared with that in the normal saline control group (P<0.05)

and there were significant differences between suramin positive control group and normal saline control group (P<0.01). Conclusion: Compared the two different extracted methods and two different positive control drugs

the ethyl acetate extracted part was better in pharmacological repetition

and with convenient methods.Also suramin positive control group was better in stability (P<0.05).So to chose the ethyl acetate extract part and suramin positive control group was exercisable.