Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC
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Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 10, Pages: 101-103(2011)
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Published:2011
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XIA Lian, CHEN Wei-wei. Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 101-103.
DOI:
XIA Lian, CHEN Wei-wei. Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 101-103.DOI:
Determination of Content of Chlorogenic Acid in Qinyi Lidan Granules by HPLC
Objective: To establish a method for determination of the content of chlorogenic acid in Qinyi Lidan granules by HPLC. Method :A HPLC method was used with welchrom C18 column(4.6 mm×250 mm
5 μm). The mobile phase was consisted of acetonitrile-0.1% phosphoric acid(9 ∶91). The UV detector wavelength was at 327 nm.The flow rate was 1 mL ·min-1.The temperature of the column was at 35 ℃. Result: The calibration curve of chlorogenic acid was linear within the range of 0.303 5-1.517 5 μg(r=0.999 7). The average recovery was 97.3%(RSD 1.9%). Conclusion: The method is simple
convenient and reliable. It is appropriate for the content determination of the preparation.