Objective: To study the inhibitory effect and mechanism of phenylpropanoid glycosides from Boschniakia rossica (PGBR) on proliferation of lung carcinoma cells. Method: MTT assay was used to examine the proliferation of A549 cells

flow cytometry was used for the determination of cell cycle distribution and cell apoptosis

TUNEL method was used for the determination of cell apoptosis

and immunohistochemical method was used for the determination of p53

Fas

Bax and Bcl-2 protein expression. Result: PGBR inhibited the proliferation of lung carcinoma cells in a time-and concentration-dependent manner

the inhibitory ratios of 200 mg ·L-1 PGBR at 12

24 and 48 h were(20.4±2.1)%

(27.1±2.0)% and (30.7±2.2)%

respectively. PGBR increased the percentage of G0/G1 phase cells from(45.3±4.1)% to (57.4±4.0)%

and increased the apoptotic ratio from(2.4±0.90)% to (7.8±1.2)% to (57.4±4.0)%

and increased the apoptotic ratio from(2.4±0.90)% to(7.8±1.2)%. Furthermore

PGBR increased the expression of p53

Fas and Bax proteins and decreased the expression of Bcl-2. Conclusion: PGBR could exert an anti-lung cancer effect via induction of cell cycle arrest and cell apoptosis.