Experimental of RG-14260 on Improving Chemosensitivity of Human Endometrial Carcinoma Cells

DENG Shou-heng; ZHOU Bao-feng; YU Xiong-jie; QU Ping; XU Hua; CAO Feng-jun

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Experimental of RG-14260 on Improving Chemosensitivity of Human Endometrial Carcinoma Cells

更新时间：2024-01-04

- Experimental of RG-14260 on Improving Chemosensitivity of Human Endometrial Carcinoma Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 7, Pages: 210-212(2011)
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- Published：2011
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DENG Shou-heng, ZHOU Bao-feng, YU Xiong-jie, et al. Experimental of RG-14260 on Improving Chemosensitivity of Human Endometrial Carcinoma Cells [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 210-212.
DOI：

DENG Shou-heng, ZHOU Bao-feng, YU Xiong-jie, et al. Experimental of RG-14260 on Improving Chemosensitivity of Human Endometrial Carcinoma Cells [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 210-212. DOI：

摘要

目的: 研究酪氨酸磷酸化抑制剂RG-14260(RG)单独或联合阿霉素、顺铂及紫杉醇对子宫内膜癌ishikawa细胞增殖的影响

为临床应用RG治疗子宫内膜癌提供理论依据。方法: 应用10 μmol ·L-1RG 与各1/2的IC50量的阿霉素(ADM)、顺铂(DDP)及紫杉醇(PTX)联合

MTT法检测细胞增殖抑制率;RT-PCR法检测RG、阿霉素、顺铂及紫杉醇对细胞表皮生长因子受体EGFRmRNA表达影响; Western blot法检测10 μmol ·L-1RG 作用6

24 h后细胞p21蛋白表达水平。结果: RG可显著降低细胞EGFR 基因mRNA水平

上调p21蛋白表达。结论: RG通过抑制EGFR信号通路

上调p21蛋白表达

增强化疗药物对子宫内膜癌细胞的杀伤作用。

Abstract

Objective: To investigate the effect of RG-14260 on the proliferation and drug sensitivity of human endometrial carcinoma line ishikawa cells in vitro. Method: Cell proliferation inhibition rate of ishikawa cells was determined using MTT method after treatment with half of the IC50of the ADR

DDP

PTX and 10 μmol ·L-1RG alone or combination for 24 h. The mRNA levels of EGFR after treatment with RG and drug alone was detected by RT-PCR. Western blot was used to measured p21 protein abundance after treatment with 10 μmol ·L-1RG for 6

24 h. Result: RG-14260 significantly decreased the mRNA expressions of EGFR and increased the abundance of p21 protein. Compared with the treatment with agents alone

combined treatment of the RG-14260 significantly increased cell proliferation inhibition rate and improved the cytotoxicity of the chemotherapeutic agents(P<0.05). Conclusion: RG-14260 can block the EGFR pathway and increase p21 protein to inhibit the proliferation and enhance the chemosensitivity of human endometrial carcinoma ishikawa cells.

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