Study on Identification of Different Parts of by Multi-steps Infrared Macro-fingerprint Methed
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Study on Identification of Different Parts of by Multi-steps Infrared Macro-fingerprint Methed
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 9, Pages: 113-117(2011)
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Published:2011
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WANG Peng, GUO Qing-mei, ZHAO Qi-tao, et al. Study on Identification of Different Parts of by Multi-steps Infrared Macro-fingerprint Methed[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 113-117.
DOI:
WANG Peng, GUO Qing-mei, ZHAO Qi-tao, et al. Study on Identification of Different Parts of by Multi-steps Infrared Macro-fingerprint Methed[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 113-117.DOI:
Study on Identification of Different Parts of by Multi-steps Infrared Macro-fingerprint Methed
Objective: The different parts of Salvia miltiorrhiza Bge f. alba was analysed by IR spectroscopy. Method: Multi-steps infrared macro-fingerprint methed(infrared spectroscopy
secondary derivative spectroscopy and two dimensional correlation infrared spectroscopy) was used to analyse the powder
ethanol-soluble and water-soluble extracts of the root
stem
leaf
flower of S. miltiorrhiza. Result: In effective component characteristics absorption area (1 800-1 200 cm-1 band) the peak intensity of the leaf was higher than root
stem and flower. In the one-dimensional spectra
except flower
the ethanol soluble extract of root
stem and leaf all appeared the adjacent quinone C=O characteristic peak absorption 1 692 cm-1 or 1 670 cm-1. In water-soluble extract
the skeleton vibration peak 1 610 cm-1 of aromatic compounds (like salvianolic acids B) in leaf and flower was more obvious than root and stem. In the secondary derivative spectra
ethanol-soluble extract and water-soluble extracts all appeared the characteristic peak of standard substance
which confirm the conclusions of one-dimensional spectrograms. In the two-dimensional correlation spectra
the automatic peak position and intensity of different parts of S. miltiorrhiza were different. Conclusion: The kinds and content of chemical composition of different part of S. miltiorrhiza were different
and the stem
the leaf and the flower were resources to be further exploited.