Objective: To develop a RP-HPLC method for simultaneous determination of two flavonoids

quercetin-3-O- -5-O-β-D-glucopyranoside(compound I) and quercetin-3-O- -5-O-β-D-glucopy-ranoside(compound II) in the seeds of Impatiens balsamina. Method: An AlltimaTM-C18 column(4.6 mm×250 mm

5 μm) was used at 35 ℃ with the mobile phases of methanol-water(containing 0.1% acetic acid) in gradient manner(0-10 min

25%-28%A

11-12 min

28%-34%A

13-20 min

34%-35%A

21-28 min

35%-41%A

29-35 min

41-25%A). The flow rate was 1.0 mL ·min-1

and the detection wavelength was 345 nm. Result: The linearity was in the range of 0.210-4.200 μg and 0.206-4.120 μg

the correlation coefficients(n=6) were 0.999 0 and 0.999 8; the average recoveries(n=6) were 98.26% and 101.73% for two flavonoids respectively. Conclusion: Determination of two flavonoids in the seeds of Impatiens balsamina was developed for the first time

and provided a reference for the quality standard of it.