Selecting of Extraction Technigues for Zishen Ningshen Decoction Based on Tranquilizing Effect

LIN Rong-feng; CHEN Jian-ping; LU Xiao-feng; HE Yao-hui; LI Yi-sheng; HUANG Xiao-qi; SHI Xu-guang; ZHANG Xi-qiang; SU Zi-ren

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Selecting of Extraction Technigues for Zishen Ningshen Decoction Based on Tranquilizing Effect

更新时间：2024-01-04

- Selecting of Extraction Technigues for Zishen Ningshen Decoction Based on Tranquilizing Effect
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 6, Pages: 220-222(2011)
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- Published：2011
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LIN Rong-feng, CHEN Jian-ping, LU Xiao-feng, et al. Selecting of Extraction Technigues for Zishen Ningshen Decoction Based on Tranquilizing Effect[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 220-222.
DOI：

LIN Rong-feng, CHEN Jian-ping, LU Xiao-feng, et al. Selecting of Extraction Technigues for Zishen Ningshen Decoction Based on Tranquilizing Effect[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 220-222. DOI：

摘要

目的: 筛选滋肾宁神方的提取工艺。方法: 通过小鼠自主活动实验及睡眠实验

对水提、醇提、水提醇沉、部分水提部分醇提等4种不同提取工艺进行筛选。结果: 水提醇沉工艺提取物可显著减少小鼠自主活动

明显延长阈下剂量戊巴比妥钠致小鼠入睡;水提工艺提取物可明显延长阈剂量戊巴比妥钠致小鼠睡眠时间。4种工艺提取物均可提高小鼠入睡率

其中水提醇沉工艺提取物对小鼠入睡率的提高最显著。结论: 选择水提醇沉工艺为滋肾宁神方的提取工艺。

Abstract

Objective: To select the extraction technigues for Zishen Ningshen decoction. Method: By the experiment of locomotor activity and sleep in mice

four extraction techniques

i.e. water-extraction

alcohol-extraction

water-extraction with alcohol-precipitation

water-extraction and partial alcohol-extraction

were evaluated. Result: The extracts of water-extraction and alcohol-precipitation and water-extraction could significantly decrease the locomotor activity

significantly prolong the sleeping time induced by the under-threshold doses pentobarbital sodium

and the extract of partial water-extraction with partial alcohol-extraction could significantly prolong the sleeping time induced by the threshold doses pentobarbital sodium; and the four extracts all could improve the sleeping rate of the mice

among which water-extraction with alcohol-precipitation extract performed best. Conclusion: The water-extraction alcohol-precipitation was selected as the extraction technique for Zishen Ningshen decoction.

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