RP-HPLC Simultaneous Determination of Mangiferin and Spinosin in Ziziphus Jujuba Mill Granules under Double UV Wavelengths

TIAN Xiao-fei; LI Qing; LIANG Ke; LIU Li; CHEN Xiao-hui; BI Kai-shun

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RP-HPLC Simultaneous Determination of Mangiferin and Spinosin in Ziziphus Jujuba Mill Granules under Double UV Wavelengths

更新时间：2024-01-04

- RP-HPLC Simultaneous Determination of Mangiferin and Spinosin in Ziziphus Jujuba Mill Granules under Double UV Wavelengths
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 11, Pages: 52-56(2011)
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- Published：2011
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TIAN Xiao-fei, LI Qing, LIANG Ke, et al. RP-HPLC Simultaneous Determination of Mangiferin and Spinosin in Ziziphus Jujuba Mill Granules under Double UV Wavelengths[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 52-56.
DOI：

TIAN Xiao-fei, LI Qing, LIANG Ke, et al. RP-HPLC Simultaneous Determination of Mangiferin and Spinosin in Ziziphus Jujuba Mill Granules under Double UV Wavelengths[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 52-56. DOI：

摘要

目的: 建立复方酸枣仁颗粒中芒果苷和斯皮诺素含量测定的反相高效液相色谱方法。方法: 采用液-液萃取法纯化样品。采用Hypersil C18色谱柱 (4.6 mm×250 mm

5 μm)

以乙腈-1%冰醋酸水系统作为流动相

梯度洗脱;波长检测程序0~25 min

258 nm

25~35 min

335 nm;流速1.0 mL · min-1;柱温30 ℃。结果: 在上述色谱条件下

测得芒果苷和斯皮诺素分别在4.320×10-3~5.200×10-2g ·L-1(r=0.999 4) 和5.200×10-3~1.040×10-1g ·L-1 (r=0.999 7) 线性关系良好;芒果苷和斯皮诺素的平均回收率 (n=9)分别为94.6%(RSD 1.9%)和95.1%(RSD 2.3%)。结论: 方法操作简便

准确

重复性良好

可用于复方酸枣仁颗粒的质量控制。

Abstract

Objective: To develop a RP-HPLC method for determination of mangiferin and spinosin in Ziziphus Jujuba Mill granules. Method: Samples were purified by Liquid-Liquid extraction.Hypersil C18(4.6 mm×250 mm

5 μm) column was used.The mobile phase consisted of acetonitrile and water with 1% glacial acetic acid;the flow rate was 1.0 mL ·min-1.Detection wavelengths were set at 258 nm

and switched to 335 nm at 25 min. Result: The linear response ranges were 4.320×10-3-5.200×102g ·L-1 (r=0.999 4) and 5.200×10-3-1.040×10-1g ·L-1 (r=0.999 7)for mangiferin and spinosin respectively.The average recoveries (n=9)of these compounds wre 94.6% (RSD 1.9%) and 95.1%(RSD 2.3%)respectively. Conclusion: A simple

accurate and reproducible method is provided for simultaneous determination of mangiferin and spinosin in Ziziphus Jujuba Mill granules by RP-HPLC.

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