Determination of Three Flavonoids In Seeds of  by HPLC

PU Yan-nan; TANG Li-ying; WANG Zhu-ju; ZHU Jie; CUI Yuan-jun; CHEN Sui-qing

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Determination of Three Flavonoids In Seeds of by HPLC

更新时间：2024-01-04

- Determination of Three Flavonoids In Seeds of by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 10, Pages: 89-91(2011)
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- Published：2011
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PU Yan-nan, TANG Li-ying, WANG Zhu-ju, et al. Determination of Three Flavonoids In Seeds of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 89-91.
DOI：

PU Yan-nan, TANG Li-ying, WANG Zhu-ju, et al. Determination of Three Flavonoids In Seeds of by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 89-91. DOI：

摘要

目的: 建立沙苑子中3个黄酮的HPLC的含量测定方法。方法: 采用高效液相色谱法

Kromasil C18色谱柱(4.6 mm×250 mm

5 μm)

流动相为甲醇-1%冰醋酸(56 ∶44)

流速为1 mL ·min-1

柱温30 ℃

检测波长为266 nm。结果: 沙苑子中毛蕊异黄酮、芒柄花素、鼠李柠檬素的线性范围依次为0.348~1.74(r=0.999 7)

0.288~1.44(r=0.999 8)

0.120~0.60(r=0.999 7)

平均回收率(n=5)分别为100.3%(RSD 0.33%)

99.8%(RSD 2.78%)

98.6%(RSD 2.91%)。结论: 该方法操作简便

结果准确

能够同时测定沙苑子药材中3个黄酮的含量

为沙苑子的质量控制和质量标准制定提供了参考依据。

Abstract

Objective: To estabish the HPLC method for determining the content of three flavonoids(calycosin

formononetin and rhamnocitrin)in the seeds of Astragali complanati. Method :An HPLC method has been developed to separate the three flavonoids on Kromasil C18(4.6 mm×250 mm

5 μm)column with methanol-1% acetic acid(56 ∶44)as mobile phase(1 mL ·min-1)

column temperature at 30 ℃ and UV detection wavelength at 266 nm. Result: The good linearity range of calycosin

formononetin and rhamnocitrin is 0.348-1.74(r=0.999 7)

0.288-1.44(r=0.999 8)

0.120-0.60(r=0.999 7). The average recovery of Calycosin

Formononetin and Rhamnocitrin are 100.3%(RSD 0.33%)

99.8%(RSD 2.78%)

98.6%(RSD 2.91%). Conclusion: The estabished method is simple

accurate and can be used for simultaneously determining the content of three flavonoids and for the the quality control of the seeds of A.complanati.

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Related Institution

Jiangxi University of Chinese Medicine

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