Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53
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Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 12, Pages: 123-126(2011)
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Published:2011
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JI Xu-ming, OU Yang-bing, WU Zhi-chun, et al. Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 123-126.
DOI:
JI Xu-ming, OU Yang-bing, WU Zhi-chun, et al. Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 123-126.DOI:
Effect of Wenxia Recipe Medicated Serum on Inducing Apoptosis ofA549/DDP Cells and Expression of Bcl-2,Bax and p53
Objective: To investigate the effect of Wenxia recipe medicated serum on inducing apoptosis of A549/DDP cells and expression of Bcl-2
Bax and p53 protein by using serum pharmacological methods in vitro. Method: Drug-containing serum was prepared and analyzed by HPLC. Furthermore
A549/DDP cells were divided into DDP group
drug-containing serum + DDP group and normal serum +DDP group.After incubated 24h
cell apoptosis was evaluated by laser flow cytometry and the expression of Bcl-2
Bax and p53 protein in cells was investigated by laser scanning confocal microscope. Result: There were ginsenoside Rb1
emodin
aconitine in drug-containing serum. Compared to DDP group
the apoptosis rate of drug-containing serum + DDP group rose to (21.82±3.27)% (P<0.05)
the expression of Bcl-2 significantly dropped to 2.81±0.02 (P<0.05) and the expression of Bax and p53 significantly rose to 2.90±0.08 and 3.58±0.09 (P<0.05) respectively. Conclusion: Wenxia recipe medicated serum could significantly induce apoptosis of A549/DDP
decrease the expression of Bcl-2 and increase the expression of Bax and p53.This study showed that Wenxia recipe might reverse the mutidrug-resistance of A549/DDP cell by inducing apoptosis.
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