Objective: To study prevention and treatment of pulmonary fibrosis by Lifei Huaxian prescription (LHP)

a formulated prescription based on traditional prescription Shengjiang San. And to investigate its influence on pulmonary function and the content of hydroxyproline in serum and in tissue. Method: One hundred Wistar rats (half male and half female)

weighing(200± 20)g

were randomly divided into 5 groups evenly (20 in each). Model of pulmonary fibrosis was established by injection of amphotericin A5 in the airway. Normal controls and model group were given the same volume of physiological saline. The dosage of LHP was 4.41 g ·kg-1 ·d-1

prednisone dosage was 1.05 mg ·kg-1 ·d-1

prednisone+LHP treatment group was given the same amount of prednisone and LHP.The experiment lasted for 7

28 d. Afterwards

animal lung function was analyzed and the serum and lung tissue hydroxyproline content was determined. Pathological method was used for morphological observation with sappanwood fine-Iraq red and Masson dyeing slice. Result: The lung tissue morphology showed that the model establishment was successful. The lung function of forced vital capacity (FVC)

largest exhaled quantity (PEF) and the first 0.25 s hard exhaled volume percentage of forced vital capacity (FEV 0.25/FVC%)

maximum volume (MVV) were investigated. Testss showed that the treatment group and model group had significant differences in lung function and serum and tissue hydroxyproline content. They were significantly improved by the combination of LHP and prednisone. Conclusion: Lifei Huaxian prescription can significantly improve pulmonary fibrosis and lung function in rats with pulmonary fibrosis

and reduce serum and tissue hydroxyproline content

especially when applied with prednisone treatment.