Study on Establishing Method to Deternine APPS Liposome Entrapment Efficiency

WU Jin-jin; ZHU Yu-qing; GE Wei-hong; LI Chang-yu; SHI Sen-lin

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Study on Establishing Method to Deternine APPS Liposome Entrapment Efficiency

更新时间：2024-01-04

- Study on Establishing Method to Deternine APPS Liposome Entrapment Efficiency
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 6, Pages: 20-23(2011)
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- Published：2011
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WU Jin-jin, ZHU Yu-qing, GE Wei-hong, et al. Study on Establishing Method to Deternine APPS Liposome Entrapment Efficiency[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 20-23.
DOI：

WU Jin-jin, ZHU Yu-qing, GE Wei-hong, et al. Study on Establishing Method to Deternine APPS Liposome Entrapment Efficiency[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 20-23. DOI：

摘要

目的: 建立猕猴桃根多糖脂质体包封率的测定方法。方法: 采用阴离子交换树脂法分离脂质体和游离APPS

采用紫外-可见分光光度法测定游离APPS含量

计算脂质体包封率。结果: 以纤维素DE-52阴离子交换树脂为填料

采用1.0 mol ·L-1NaCl溶液洗脱

游离APPS的回收率99.21%

空白脂质体的回收率0.07%;线性范围11.66~58.29 mg · L-1(r=0.999 4)

日内和日间精密度均符合要求(RSD均小于2%)

方法平均回收率99.28%

RSD 0.66%;APSP脂质体的平均包封率70.16%。结论: 此法简便、可行

可用于猕猴桃根多糖脂质体包封率的测定。

Abstract

Objective: The method was established to determine the entrapment efficiency for the APPS liposome. Method: Anion exchange resin was used to separate the liposome and free APPS; UV-visible spectrophotometry was used to determine the content of APPS

and liposome encapsulation efficiency was calculated. Result: The method established was as follows: the anion-exchange resin was used with 1.0 mol ·L-1NaCl as eluent; the free APPS recovery was 99.21%

liposome recovery was 0.07% with the linear range of 11.66-58.29 mg·L-1(r=0.999 4). The intra- and inter-day precisions (RSD) could meet the requirements (within 2.0%); the average recovery was 99.28%

RSD was 0.66%. The encapsulation efficiency of APPS liposome was 70.16% on average. Conclusion: This method is simple and feasible

and can determine the entrapment efficiency for the APPS liposome.

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