HPLC Determination of Chlorogenic Acid,Forsythoside,Rutin and Phillyrin in  Fowers and Leaves

ZHANG Fei; TIAN Su; LV Ming-xia; HE Tong-sen; LI Yuan; DING Yue-xin; CUI Tong

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HPLC Determination of Chlorogenic Acid,Forsythoside,Rutin and Phillyrin in Fowers and Leaves

更新时间：2024-01-04

- HPLC Determination of Chlorogenic Acid,Forsythoside,Rutin and Phillyrin in Fowers and Leaves
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 9, Pages: 103-106(2011)
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- Published：2011
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ZHANG Fei, TIAN Su, LV Ming-xia, et al. HPLC Determination of Chlorogenic Acid,Forsythoside,Rutin and Phillyrin in Fowers and Leaves[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 103-106.
DOI：

ZHANG Fei, TIAN Su, LV Ming-xia, et al. HPLC Determination of Chlorogenic Acid,Forsythoside,Rutin and Phillyrin in Fowers and Leaves[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(9): 103-106. DOI：

摘要

目的: 建立同时测定绿原酸、连翘酯苷、芦丁、连翘苷含量的高效液相色谱测定方法;比较连翘花、不同生长时期连翘叶、连翘药材中的含量差异。方法: 采用高效液相色谱(HPLC)法

以Supelcosil C18柱(4.6 mm ×250 mm

5 μm)为色谱柱

甲醇-0.8%醋酸水溶液为流动相梯度洗脱

流速1.0 mL ·min-1

检测波长280 nm

柱温25 ℃。结果: 绿原酸在0.125~12.500 μg、连翘酯苷在0.125~12.500 μg、芦丁在0.175~17.500 μg、连翘苷在0.125~12.500 μg线性关系良好

平均加样回收率为98.43%~99.97%

RSD<5%(n=6)。结论: 该方法准确可靠

结果稳定

可用于连翘样品中绿原酸、连翘酯苷、芦丁和连翘苷的同时测定

且各活性成分在连翘花、不同生长时期连翘叶及连翘药材的含量存在差异。

Abstract

Objective: The HPLC method was established for determination of chlorogenic acid

forsythoside

rutin and phillyrin in Forsythia suspense flowers and leaves. Method: The separation was performed on Supelcosil C18 column (4.6 mm ×250 mm

5 μm) at 25 ℃ using methanol-0.8% aqueous acetic acid as the mobile phase at a flow rate of 1.0 mL ·min-1

the detection wavelength was 280 nm. Result: The standard curve was linear within the concentration range of 0.125-12.500 μg for chlorogenic acid

0.125-12.500 μg for forsythoside

0.175-17.500 μg for rutin and 0.125-12.500 μg for phillyrin. The average recovery was 99.43%-99.97%

respectively. The RSD was less than 5%. Conclusion: The method is sensitive and accurate. It can be used for determination of chlorogenic acid

forsythoside

rutin and phillyrin in Forsythia suspense.

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