Effect of Jingyuankang Capsule on Expression of Apoptosis-related Genesin Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression
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Effect of Jingyuankang Capsule on Expression of Apoptosis-related Genesin Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 12, Pages: 119-122(2011)
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Published:2011
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JIANG Li-feng, SANG Feng, JIANG Shi-qing, et al. Effect of Jingyuankang Capsule on Expression of Apoptosis-related Genesin Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 119-122.
DOI:
JIANG Li-feng, SANG Feng, JIANG Shi-qing, et al. Effect of Jingyuankang Capsule on Expression of Apoptosis-related Genesin Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(12): 119-122.DOI:
Effect of Jingyuankang Capsule on Expression of Apoptosis-related Genesin Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression
以深入研究、探讨该药的作用机制。 方法: 60只小鼠分为空白组、模型组、阳性对照利可君32 mg ·kg-1组和贞芪扶正3 g ·kg-1组、精元康4 g ·kg-1组
以环磷酰胺100 mg ·kg-1
ip连续3 d造模
同时各组开始ig连续7 d
实时荧光定量PCR检测骨髓抑制模型小鼠骨髓细胞表达Caspase-3
Fas
FasL mRNA的情况。 结果: Caspase-3
Fas
FasL mRNA的表达水平精元康胶囊组最低
模型组表达的 1 2 与模型组比较有显著差异
与空白组比较无明显差异。 结论: 精元康胶囊降低促凋亡基因Caspase-3
Fas
FasL的表达
对骨髓细胞凋亡相关通路产生影响
可能是精元康胶囊对化疗致骨髓抑制影响的重要机制。
Abstract
Objective: To observe the effect of Jingyuankang capsule on the expression of apoptosis-related genes Caspase-3
Fas and FasL mRNA in bone marrow cells and make a study of the mechanism of the medicine. Method: Sixty mice were randomized into 5 groups:control group
model group
positive control group(Leucogen
32 mg ·kg-1)
Zhenqi Fuzheng group(3 g ·kg-1)
Jingyuankang group(4 g ·kg-1). The model was induced by ip cyclophosphamide(100 mg ·kg-1) for 3 days. Mean while the above treatments were given for 7 days. The expression of Caspase-3
Fas and FasL mRNA in bone marrow cells of model mice with myelosuppression was measured by real-time fluorescence quantitative PCR. Result: The Jingyuankang group had the lowest expression of Caspase-3
Fas and FasL mRNA
compared with that of the model group
the difference was significant;while compared with the control group
there was no significant differences. Conclusion: These results confirmed preliminarily that Jingyuankang capsule could decrease the expression of apoptosis-related genes Caspase-3
Fas and FasL
through affecting the apoptosis-related pathway to prevent the blood cells from apoptosis caused by chemotherapy.This might be an important mechanism of Jingyuankang capsule treating the chemotherapy-induced myelosuppression.
Effect of Jingyuankang Capsule on Expression of Bcl-2, Bax mRNA in Bone Barrow Cells of Model Mice of Chemotherapy-induced Myelosuppression
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Related Institution
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