Objective: To separate and purify polysaccharides from root of Cherokee Rose (Jinyinggen

JYG) in Chinese

and investigate its antitumor activity in vivo. Method :Water extracting and ethanol precipitating methods were applied to extract polysaccharide from root of Cherokee Rose. Sevage method was used to remove protein

and macro porous resin absorption was used for decolorization. Mice tumor model was established with transplantion of S180 solid tumor or ascites tumor. And the S180- ascites mice were divided into four groups: control(NS)

JYG-Low

medium

high (50

100

200 mg ·kg-1). For the mice model with transplanted S180 solid tumor

the mice were divided into eight groups: control(NS)

5-Fu(20 mg ·kg-1)

JYG-Low

medium

high (50

100

200 mg ·kg-1)

JYG-Low

medium

high (50

100

200 mg ·kg-1)+5-Fu(20 mg ·kg-1). Intraperitoneal injection was used to be the major route of administration. The effects of different groups on the inhibitory rate

the proliferation rate

WBC

thymus index(TI) and spleen index(SI) were observed after the mice were sarcrified. Result: The sugar content of JYG polysaccharides was 85.43%.The life-prolonging rates of JYG polysaccharides (low

medium

high) were 3.07%±2.35%

10.11%±4.32%

34.3%±8.31% respectively. There was only significant differences between high dose group and control group. The antitumor effect of JYG polysaccharides-low

meddian

high doses combinated with 5-Fu were superior to 5-Fu

the inhibitory rate were 62.74%±4.35%

67.92%±3.24%

78.79%±6.23% respectively. Compared with 5-Fu alone

the drug combination could increase WBC count

TI

SI and the IL-2 level

but had no effect on TNF-α. Conclusion : The single use of JYG polysaccharides was not effect to inhibit the growth of S180 tumor

but the combination of JYG polysaccharide with 5-Fu had a coordinated effect with better efficacy and lower toxicity.