Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells

ZHANG Shi-jun; ZHANG Zhi-wei; CHEN Zhao-ni; JIAO Yang; LI Yong-wen; HUANG Ren-bin

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Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells

更新时间：2024-01-04

- Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 3, Pages: 135-138(2011)
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- Published：2011
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ZHANG Shi-jun, ZHANG Zhi-wei, CHEN Zhao-ni, et al. Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 135-138.
DOI：

ZHANG Shi-jun, ZHANG Zhi-wei, CHEN Zhao-ni, et al. Influence of Compound Liuyueqing on Proliferation and Extracellular Matrix of Hepatic Stellate Cells[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 135-138. DOI：

摘要

目的: 研究复方六月青(CLYQ)对肝星状细胞(HSC)增殖和细胞外基质(ECM)的影响。方法: 采用四甲基噻唑蓝(MTT)法检测CLYQ对HSC的毒性。采用酶联免疫吸附实验(ELISA)法检测HSC上清液羟脯氨酸(Hyp)、透明质酸(HA)和金属蛋白酶组织抑制因子-1(TIMP-1)。结果: CLYQ体外对HSC的 TC50=6.191 g ·L-1

TC0=3.504 g ·L-1

CLYQ抑制HSC的增殖。CLYQ各剂量组均可显著降低HSC细胞上清液Hyp

TIMP-1含量(P<0.05

P<0.01)

呈现量效反应关系。结论: CLYQ通过抑制HSC的增殖

从而使ECM合成减少

肝纤维化减轻。

Abstract

Objective: To study the influence of CLYQ on proliferation and extracellular matrix(ECM) of hepatic stellate cells(HSC). Method: Cytotoxicity caused by CLYQ was detected by MTT assay. The contents of hydroxyproline (Hyp)

hyaluronic acid (HA) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in HSC cell supernate were detected by enzyme linked immunoabsorbent assay (ELISA). Result: TC50 is 6.191 g ·L-1

and TC0 is 3.504 g ·L-1. CLYQ could inhibit the proliferation of HSC. The various concentration group of CLYQ could degrade significantly the contents of Hyp

HA and TIMP-1 in cell supernate (P<0.05

P<0.01)

presenting the dose-effect relationship. Conclusion: CLYQ can inhibit the proliferation of HSC

so that reducing the synthesis of ECM and hepatic fibrosis.

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