Study on Extraction Technology and Antioxidant Activity of Theabrownine from Oolong Tea

ZHOU Xiang-jun; GAO Yi-xia; YUAN Yi-jun; YANG Hong-juan; ZHANG Ji

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Study on Extraction Technology and Antioxidant Activity of Theabrownine from Oolong Tea

更新时间：2024-09-23

- Study on Extraction Technology and Antioxidant Activity of Theabrownine from Oolong Tea
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 4, Pages: 36-40(2011)
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- Published：2011
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ZHOU Xiang-jun, GAO Yi-xia, YUAN Yi-jun, et al. Study on Extraction Technology and Antioxidant Activity of Theabrownine from Oolong Tea[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 36-40.
DOI：

ZHOU Xiang-jun, GAO Yi-xia, YUAN Yi-jun, et al. Study on Extraction Technology and Antioxidant Activity of Theabrownine from Oolong Tea[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 36-40. DOI：

摘要

以乌龙茶为原料

研究了不同因素对乌龙茶茶褐素得率的影响

测定了茶褐素中蛋白质、总糖、总酚及总黄酮的含量

同时初步探讨了茶褐素的抗氧化活性。结果表明

各因素对茶褐素得率的影响依次为:提取次数>提取时间>提取温度>料液比;最佳提取工艺为

料液比1 ∶10

提取次数3次

提取温度70 ℃

提取时间20 min

在此条件下

茶褐素得率为9.23%。茶褐素主要成分为蛋白质(21.47%)、糖(28.28%)和总酚(11.19%)。抗氧化活性结果表明

乌龙茶茶褐素对羟自由基、超氧阴离子自由基、DPPH自由基均具有一定的清除效果

且随着浓度的增大清除率增加。

Abstract

The effects of different factors on extraction ratio of theabrownine from Oolong tea were studied

and at the same time antioxidant activity of the resulting product

the content of protein

polysaccharides as well as the total phenols were determined.The optimum extraction conditions were as follows:solid-liquid ratio 1 ∶10

extraction temperature at 70 ℃

extraction 3 times and extraction for 20 min.Under these conditions

the content of theabrownine was 9.23%.Theabrownine are mainly made up of protein 21.47%

polysaccharides 28.28% and total phenols 11.19%

respectively.Theabrownine had scavenging ability for hydroxyl radical

superoxide anion radical and DPPH radical

and its scavenging ability increased with the growth of theabrownine content.

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Related Author

ZHOU Xiang-jun1

GAO Yi-xia1

YUAN Yi-jun1

YANG Hong-juan1

ZHANG Ji1

Chuan-hong LUO

Sheng-jie HUANG

Qi-qi HU

Related Institution

Department of Life Science,Northwest Normal University

College of Life Science and Chemistry,Tianshui Normal University

School of Pharmacy，State Key Laboratory of Characteristic Chinese Drug Resources in Southwest China，Chengdu University of Traditional Chinese Medicine（TCM）

State Key Laboratory of Biological Evaluation of TCM Quality，National Administration of TCM， Sichuan Academy of Chinese Medicine Sciences

Sanajon Pharmaceutical Group

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