Determination of Diosgenin in  by HPLC-ELSD

PAN Hai-feng; HONG Xia; LI Yan-rong

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Determination of Diosgenin in by HPLC-ELSD

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- Determination of Diosgenin in by HPLC-ELSD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 8, Pages: 119-121(2011)
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- Published：2011
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PAN Hai-feng, HONG Xia, LI Yan-rong. Determination of Diosgenin in by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 119-121.
DOI：

PAN Hai-feng, HONG Xia, LI Yan-rong. Determination of Diosgenin in by HPLC-ELSD[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(8): 119-121. DOI：

摘要

目的: 建立高效液相色谱蒸发光散射检测(HPLC-ELSD)法测定穿山龙中薯蓣皂苷元的含量

并与2005年版《中国药典》方法紫外检测器(DAD)测定的结果进行比较。方法: Agilent EclipseXDB-C18柱(4.6 mm×150 mm

5 μm)

流动相甲醇-水(95 ∶5)

柱温40 ℃;ELSD检测器漂移管温度为70 ℃

载气压力367.5 Pa

Gain7

Filter1;进样量10 μL。结果 :薯蓣皂苷元在2.60~20.8 μg线性关系良好(r=0.999 94)

平均回收率为97.3%

RSD 1.94%。结论 :该方法简便

灵敏

准确性高

可作为穿山龙中薯蓣皂苷元含量测定的方法;与DAD相比

该法所得图谱基线平整

检测灵敏度高

样品分离度好

测得样品含量偏高。

Abstract

Objective: To develop an HPLC-ELSD method for the determination of diosgenin in Dioscorea nipponica and comparison of content by ELSD and DAD. Method: An Agilent Eclipse XDB-C18(4.6 mm×150 mm

5 μm) was used with the mobile phase of methanol-water(95 ∶5) at 40 ℃. Evaporated light scattering detector was used. The drift tube temperature was at 70 ℃ and the pressure of nebulizing gas was 367.5 Pa

gain was 7

filter was 1.The injected volume was 10 μL. Result: The linear calibration curve was obtained in the ranges of 2.60-20.8 μg for diosgenin(r=0.999 94). The average recovery was 97.3% with RSD of 1.94%. Conclusion: This method is simple

sensitive and accurate. It can be used for the quality control of D. nipponica Makino. This method achieves more flat baseline

higher sensitivity

better resolution and higher content than DAD.

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Related Author

ZHOU Nong

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YANG Li-yun

XU Ping

JIANG Bei

WANG Zhi-hong

LI Xue-ting

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Related Institution

College of Pharmacy,Dali University,Dali

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

School of Pharmacy，Henan Provincial Engineering Technology Research Center for Quality Control and Evaluation of Traditional Chinese Medicine， Henan University of Chinese Medicine

School of Pharmacy，Shandong University of Traditional Chinese Medicine

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