Uptake of Isoliquiritigenin in Caco-2 Cell Monolayer

ZHANG Mei-ling; WANG Xin-chun; HUANG Zhi-qiao; HUANG Chuan-sheng; YUAN Yong

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Uptake of Isoliquiritigenin in Caco-2 Cell Monolayer

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- Uptake of Isoliquiritigenin in Caco-2 Cell Monolayer
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 4, Pages: 134-136(2011)
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- Published：2011
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ZHANG Mei-ling, WANG Xin-chun, HUANG Zhi-qiao, et al. Uptake of Isoliquiritigenin in Caco-2 Cell Monolayer[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 134-136.
DOI：

ZHANG Mei-ling, WANG Xin-chun, HUANG Zhi-qiao, et al. Uptake of Isoliquiritigenin in Caco-2 Cell Monolayer[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 134-136. DOI：

摘要

目的 :研究异甘草素(ISL)在Caco-2细胞的摄取特性。方法 :以Caco-2细胞单层模型研究异甘草素的细胞摄取规律。采用高效液相色谱法测定细胞中ISL浓度

考察时间、pH值、药物浓度及抑制剂对Caco-2细胞摄取ISL的影响。结果 :ISL在Caco-2细胞中的摄取呈时间依赖性;ISL在4~15 mg ·L-1浓度范围内的摄取呈线性增加

符合被动扩散过程;pH 8.0条件下药物的细胞摄取量(0.48±0.006) μg ·mg-1显著低于pH 5.0药物的细胞摄取量(0.98±0.03) μg ·mg-1(P<0.01);加入维拉帕米

叠氮化钠及2

4-二硝基酚后

与对照组相比

ISL的细胞摄取量显著高

分别为(1.19±0.030)μg.mg-1(P<0.01)

(1.10±0.004) μg ·mg-1(P<0.05)

(1.17±0.020) μg ·mg-1(P<0.01)。结论 :ISL的细胞摄取机制主要是被动转运;P-糖蛋白参与了ISL的转运过程。

Abstract

Objective: To observe uptake process of Isoliquiritigenin (ISL) in Caco-2 cell. Method: The concentration of ISL in cells was detected by HPLC and the mechanism of ISL absorption in cells was explored by studying the time

drug concentration and inhibitors on the uptake of ISL in cells. Result: ISL in Caco-2 cells uptake was time-dependent. ISL at 4-15 mg ·L-1concentration range of uptake increased linearly

consistent with passive diffusion process. The drug intake amount (0.48±0.006) μg ·mg-1 under pH 8.0 was significantly lower than pH 5.0 (0.98 ± 0.03) μg ·mg-1 (P<0.01).Compared with the control group

ISL cell uptake was significantly higher after additional treatment with verapamil(1.19 ± 0.030) μg ·mg-1

(P<0.01)

sodium azide (1.10 ± 0.004) μg ·mg-1(P<0.05)and 2

4-dinitrophenol(1.17 ± 0.020) μg ·mg-1(P<0.01). Conclusion: The mechanism of ISL absorption in cells mainly was passive transport and P-glycoproteins participates in the conveying process of ISL in Caco-2 cells.

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Related Author

WANG Xin-chun1

HUANG Zhi-qiao1

HUANG Chuan-sheng1

YUAN Yong1

ZHANG Mei-ling1

WANG Xin-chun

HUANG Chuan-sheng3

Related Institution

Pharmacy of College,Shihezi University

The First Affiliated Hospital of Medical College

Institute for Drug Control Shihezi City

First Affiliated Hospital of Medical College,Shihezi University

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