HPLC Fingerprint Spectrum of Radix Alangii Preparata

YU Qi-rong; HE Fang; GAO Bai-li; HU Yu-xia

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HPLC Fingerprint Spectrum of Radix Alangii Preparata

更新时间：2024-01-04

- HPLC Fingerprint Spectrum of Radix Alangii Preparata
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 6, Pages: 117-120(2011)
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- Published：2011
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YU Qi-rong, HE Fang, GAO Bai-li, et al. HPLC Fingerprint Spectrum of Radix Alangii Preparata[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 117-120.
DOI：

YU Qi-rong, HE Fang, GAO Bai-li, et al. HPLC Fingerprint Spectrum of Radix Alangii Preparata[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 117-120. DOI：

摘要

目的: 建立制八角枫高效液相指纹图谱。方法 :采用高效液相色谱法

色谱柱采用Alltech Apollo C18(4.6 mm×250 mm

5 μm)

以乙腈-缓冲液(缓冲液:0.2 g庚烷磺酸钠、3.5 g磷酸二氢钾定容至1000 mL)作为流动相梯度洗脱。结果: 建立了制八角枫的HPLC指纹图谱

方法学考察符合指纹图谱技术要求。结论: 该方法稳定、准确

可靠

为制八角枫质控指标的制定提供了科学依据。

Abstract

Objective: To establish the HPLC fingerprint of the pieces of Radix Alangii Preparata. Method: Using the high performance liquid chromatography

the chromatographic column was Alltech Apollo C18 (4.6 mm × 250 mm

5 μm) and acetonitrile-buffer (buffer: 0.2 g heptane sulfonate

3.5 g potassium dihydrogen phosphate volume to 1 000 mL)was selected as mobile phase gradient elution were adopeted. Result :Established HPLC fingerprint of R Alangii Preparata

and the results of methodological studymet the technical requirements for fingerprinting. Conclusion: The method is stable

accurate

and reliable

to provide a scientific basis of quality control standard for the R.Alangii Preparata.

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