Studies on Extraction and Purification Process of Analgesic and Hemostatic Bioactive Portion from

HAO Yan-jun; SANG Yu-li

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Studies on Extraction and Purification Process of Analgesic and Hemostatic Bioactive Portion from

更新时间：2024-01-04

- Studies on Extraction and Purification Process of Analgesic and Hemostatic Bioactive Portion from
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 11, Pages: 19-22(2011)
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- Published：2011
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HAO Yan-jun, SANG Yu-li. Studies on Extraction and Purification Process of Analgesic and Hemostatic Bioactive Portion from [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 19-22.
DOI：

HAO Yan-jun, SANG Yu-li. Studies on Extraction and Purification Process of Analgesic and Hemostatic Bioactive Portion from [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(11): 19-22. DOI：

摘要

目的: 对独一味镇痛止血有效部位的富集工艺进行研究。方法: 采用二甲氨基苯甲醛法测定总环烯醚萜含量、HPLC测定8-epideoxyloganic acid含量

以正交试验法和单因素试验法筛选提取工艺和大孔吸附树脂分离条件。结果: 最佳提取工艺为用10倍量60%乙醇渗漉提取

浸泡36 h

渗漉速度5 mL ·min-1 ·kg-1。纯化工艺为将提取物上聚酰胺柱

水洗脱部分上XDA-7大孔吸附树脂

收集30%醇洗脱部分。结论: XDA-7型树脂对独一味中环烯醚萜苷的富集能力优于其他树脂。洗脱溶剂30%乙醇能较好地洗脱环烯醚萜苷类成分。

Abstract

Objective: To study the preparation process of extraction and purification for analgesic and hemostatic bioactive portion from Lamiophlomis rotata. Method: Determination of the total iridoid glycosides and 8-epideoxyloganic acid were performed by dimethylaminobenzaldehyde method and HPLC respectively. By Using single factor and orthogonal experiments

the best extraction and purification process with macroporous resin was optimized. Result: The best extraction was established as follows: solvent of 60% ethanol

soaking 36 hours

elution volume was 10 times of materials

percolation rate of 5mL ·min-1kg-1. The purification process was as follows: the ethanolic extract should be separated by polyamide. The water elution was separated with XDA-7 macroporous resin and collecting the 30% ethanolic extract. Conclusion: XDA-7 macroporous resin has better ability of absorption and anti-absorption for iridoid glycosides and 30% ethanol was the best solvent for anti-absorption.

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Studies on extraction and purification process of the analgesic and hemostatic bioactive portion from Lamiophlomis rotata (Benth.) Kudo

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