Purification of Total Saponins from Panax Ginseng by HPD Macroporous Resins
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Purification of Total Saponins from Panax Ginseng by HPD Macroporous Resins
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 6, Pages: 6-9(2011)
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Published:2011
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HUANG Li-xin, XIONG You-wen, ZHANG Qi-yun, et al. Purification of Total Saponins from Panax Ginseng by HPD Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 6-9.
DOI:
HUANG Li-xin, XIONG You-wen, ZHANG Qi-yun, et al. Purification of Total Saponins from Panax Ginseng by HPD Macroporous Resins[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 6-9.DOI:
Purification of Total Saponins from Panax Ginseng by HPD Macroporous Resins
Objective: To study the optimum methods of extracting and refining ginsenosides from red ginseng. Method: The ability of extracting and refining ginsenosides was compared in different types of macroporous resin and different technological conditions with total amount of ginsenosides as index. Result: The HPD-400 macroporous resin was best for separating and purifying total saponins in the following technological conditions: the sample concentration was 1.7 g ·L-1
pH 4.68
the flow rate was 7.5 BV ·h-1
the eluting reagent was 80% ethanol (4 times the volume of column) and eluting velocity was 7.5 BV ·h-1. Conclution : The technological condition will provide bases for the preparation technique of Traditional Chinese Medicine containing ginsenosides.