Determination of Content of Polysaccharides in Prunus Mume by Phenol-sulfuric Method
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Determination of Content of Polysaccharides in Prunus Mume by Phenol-sulfuric Method
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 6, Pages: 107-109(2011)
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Published:2011
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ZHANG Lu, WENG Li-dong, LIU Li, et al. Determination of Content of Polysaccharides in Prunus Mume by Phenol-sulfuric Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 107-109.
DOI:
ZHANG Lu, WENG Li-dong, LIU Li, et al. Determination of Content of Polysaccharides in Prunus Mume by Phenol-sulfuric Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 107-109.DOI:
Determination of Content of Polysaccharides in Prunus Mume by Phenol-sulfuric Method
在此波长处溶液的吸收度与葡萄糖含量呈良好的线性关系;线性范围0.02~0.2 g ·L-1(r=0.999 8)
加样回收率100.38%
RSD 0.892%;乌梅中多糖含量2.51%。 结论: 苯酚-硫酸显色法操作简便
结果准确
为乌梅多糖的进一步研究奠定良好的基础。
Abstract
Objective: To establish an analysis method for the determination of the content of polysaccharides from Prunus mume. Method: Using Phenol-sulfuric Method to determination of the content of polysaccharides for P.mume. Result: The absorbance was determined at 488 nm. The linear curves was fine between the concentration rang of 0.02-0.2 g ·L-1 (r=0.999 8)
the average recovery was 100.38%
the RSD was 0.892%.The content of polysaccharide in P.mume was 2.51%. Conclusion: Phenol-sulfuric method is a simple operation process with accurate result
laying a foundation for further study on polysaccharides in P.mume.
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