Studies on HPLC Fingerprint of Huanglian Jiedu Decoction and Quantitative Analysis of Index Components
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Studies on HPLC Fingerprint of Huanglian Jiedu Decoction and Quantitative Analysis of Index Components
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 10, Pages: 67-70(2011)
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Published:2011
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PENG Juan, GUO Na, FAN Bin, et al. Studies on HPLC Fingerprint of Huanglian Jiedu Decoction and Quantitative Analysis of Index Components[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 67-70.
DOI:
PENG Juan, GUO Na, FAN Bin, et al. Studies on HPLC Fingerprint of Huanglian Jiedu Decoction and Quantitative Analysis of Index Components[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(10): 67-70.DOI:
Studies on HPLC Fingerprint of Huanglian Jiedu Decoction and Quantitative Analysis of Index Components
Objective: To develop a method for HPLC fingerprint of Huanglian Jiedu decoction and quantitative analysis of index components. It will be used for quality control of Huanglian Jiedu decoction. Method :HPLC-DAD was applied on an C18 HPLC column and a gradient elution program to develop a HPLC fingerprint of Huanglian Jiedu decoction.The method validation of quantitative analysis of index components was fulfilled. Result: HPLC fingerprint of Huanglian Jiedu decoction was developed including 18 compound peaks whose resolution was beyond 1.0 and 7 index components concentration in Huanglian Jiedu decoction was determined. Conclusion: The method accurate
convenient and rapid has been applied for quality control of Huanglian Jiedu decoction.