Determination Content of Hyperin in  Choisy

YE Lv-ping; LIU Xiao-yi; LU Hai-peng; LIAO Hou-zhi; WEI Zuo-gan

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Determination Content of Hyperin in Choisy

更新时间：2024-01-04

- Determination Content of Hyperin in Choisy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 7, Pages: 78-79(2011)
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- Published：2011
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YE Lv-ping, LIU Xiao-yi, LU Hai-peng, et al. Determination Content of Hyperin in Choisy [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 78-79.
DOI：

YE Lv-ping, LIU Xiao-yi, LU Hai-peng, et al. Determination Content of Hyperin in Choisy [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(7): 78-79. DOI：

摘要

目的: 建立赶山鞭中金丝桃苷的含量测定方法。方法: 采用VP-ODS C18 (4.6 mm×150 mm

5 μm)色谱柱

以乙腈-0.1%磷酸溶液(14 ∶86)为流动相

流速1.0 mL ·min-1

检测波长为360 nm为HPLC检测条件测量7批赶山鞭含量。结果: 赶山鞭中的金丝桃苷含量侧定方法在0.099 8~0.998 0时进样量与峰面积的线性关系良好(r=0.999 8)

平均回收率为100.11%

RSD 1.20%。结论: 所建立的方法准确、灵敏、重复性好

可用于赶山鞭的质量控制。

Abstract

Objective: To establish the determination method of hyperin content in Hypericum attenuatum Choisy. Method: VP-ODS Cl8(4.6 mm×150 mm

5 μm)colmnn was used

the mobile phase was acetonitrile-0.1% phosphoric acid water(16 ∶84);with the flow rate as 1.0 mL ·min-1

the detection wavelength was at 360 nm. Result: The linear relation of hyperin was good in the range from 0.099 8 to 0.998 0 μg.The average recovery was 100.11%

RSD 1.20%. Conclusion :The method established is accurate

sensitive and good in reproducibility.It can be applied to the qualitycontrol of H.attenuatum

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Related Institution

College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine

Henan University of Chinese Medicine

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Hubei Institute for Food and Drug Control

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