Effect of 'Traditional Chinese Medicine Prescription for Reinfocing Pi Qi’ on Expression of Kinase of Nuclear Factor-kappa B Inhibitor  in Asthmatic Rats

MIN Guang-yan; WANG Yan-jie; LIU Chun; ZHAO Dan-yu; CAO Yang; GUO Juan-fu

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Effect of 'Traditional Chinese Medicine Prescription for Reinfocing Pi Qi’ on Expression of Kinase of Nuclear Factor-kappa B Inhibitor in Asthmatic Rats

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- Effect of 'Traditional Chinese Medicine Prescription for Reinfocing Pi Qi’ on Expression of Kinase of Nuclear Factor-kappa B Inhibitor in Asthmatic Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 4, Pages: 121-124(2011)
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- Published：2011
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MIN Guang-yan, WANG Yan-jie, LIU Chun, et al. Effect of 'Traditional Chinese Medicine Prescription for Reinfocing Pi Qi’ on Expression of Kinase of Nuclear Factor-kappa B Inhibitor in Asthmatic Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 121-124.
DOI：

MIN Guang-yan, WANG Yan-jie, LIU Chun, et al. Effect of 'Traditional Chinese Medicine Prescription for Reinfocing Pi Qi’ on Expression of Kinase of Nuclear Factor-kappa B Inhibitor in Asthmatic Rats[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(4): 121-124. DOI：

摘要

目的 :探讨补脾益气方对哮喘模型大鼠肺组织核因子Kappa B(NF-κB)抑制剂的激酶β(the kinase of nuclear factor-kappa B inhibitor β

IKKβ)的调控作用及对白介素8(IL-8)含量的影响。方法 :SPF级雄性Wistar大鼠随机分为对照组(A)、哮喘组(B)、补脾益气方治疗组(C)。卵蛋白致敏和激发复制出哮喘模型

按照10 g ·kg-1体重给予C组大鼠中药ig 1次/d

共21d。采用逆转录聚合酶链反应(RT-PCR)检测肺组织IKKβ的mRNA表达水平

采用免疫组化检测肺组织IKKβ的蛋白表达水平

采用酶联免疫吸附法(ELISA)测定支气管肺泡灌洗液IL-8的含量。结果 :①肺组织IKKβ的mRNA的表达:B组显著高于A组(P＜0.01);C组显著低于B组(P＜0.05)。②肺组织IKKβ的蛋白表达水平:B组显著高于A组(P＜0.01);C组显著低于B组(P＜0.01)。③肺泡灌洗液IL-8含量:B组显著高于A组(P＜0.01);C组显著低于B组(P＜0.01)。结论 :哮喘组大鼠肺组织IKKβ mRNA和蛋白的表达水平显著增强

补脾益气方能有效抑制其表达

降低肺泡灌洗液IL-8含量

证明补脾益气方能有效抑制IKKβ信号转导的途径。

Abstract

Objective: To investigate the effect of "TCM prescription for Reinfocing Pi Qi"on regulating the kinase of nuclear factor-kappa B inhibitor β(IKKβ) and the level of interleukin 8 (IL-8) in asthmatic rats’lung tissue. Method: SPF male wistar rats were randomly divided into normal control group(A)

asthmatic group(B)and therapeutic group of "TCM prescription for reinfocing Pi Qi"(C).Asthmatic model was established via sensitizing and provoking ovalbumin.Rats of the C group were taken medicine ig once a day in 10 g ·kg-1 for 21 days.The protein expression level of mRNA of IKKβ in lung tissue was determined by Polymerase Chain Reaction (RT-PCR);the level of interleukin eight (IL-8) was detected in bronchoalveolar lavage fluid (BALF) by enzymes linked immunosorbent assay(ELISA). Result: ① The expression of mRNA of IKKβ in lung tissue in B group was significantly higher than that in A group and C group(P＜0.01 and P＜0.05 respectively).②The expression of protein of IKKβ in lung tissue in B group was significantly higher than that in A group and C group(P＜0.01).③ The level of IL-8 in BALF in B group was significantly higher than that in A group and C group(P＜0.01). Conclusion: "TCM prescription for reinfocing Pi Qi" inhibits IKKβ expression and reduce the level of IL-8.

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Related Author

MIN Guang-yan

WANG Yan-jie

LIU Chun

ZHAO Dan-yu

CAO Yang

GUO Juan-fu

YANG Shuo

LI Haiyan

Related Institution

Department of Biochemistry and Molecular Biology,Liaoning University of Traditional Chinese Medicine

Institute of Information on Traditional Chinese Medicine，China Academy of Chinese Medical Sciences

Institute of Basic Research in Clinical Medicine，China Academy of Chinese Medical Sciences

Henan Provincial Hospital of Traditional Chinese Medicine（TCM）/Second Affiliated Hospital of Henan University of TCM

Putuo Hospital， Shanghai University of TCM

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