Objective: To study the HPLC fingerprints and to establish a sensitive and specific method for controlling the quality of Hibiscus Mutabilis. Method: HPLC method was set up by Waters Xbridge C18column (4.6 mm×250 mm

5 μm) with gradient elution; the mobile phase was comprised of acetonitrile-1% acetic acid solution

the UV detection wavelength was at 370 nm with a flow rate of 1.0 mL ·min-1

and column temperature was at 25 ℃. Result: There were 10 common peaks in the fingerprints of H.mutabilis based on 10 habitats. Sum of peak areas was larger than 90% of total area. As compared with standard sample

rutin

hyperin and quercitrin were checked out. Conclusion: The method is reliable

accurate and can be used as a quality control method for H.mutabilis.