Determinate Concentration of Methotrexate in Acute Lymphoblasticleukemia Children by HPLC

LIU Wen; DENG Nan; DAI Ying-chun

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Determinate Concentration of Methotrexate in Acute Lymphoblasticleukemia Children by HPLC

更新时间：2024-01-04

- Determinate Concentration of Methotrexate in Acute Lymphoblasticleukemia Children by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 3, Pages: 108-110(2011)
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- Published：2011
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LIU Wen, DENG Nan, DAI Ying-chun. Determinate Concentration of Methotrexate in Acute Lymphoblasticleukemia Children by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 108-110.
DOI：

LIU Wen, DENG Nan, DAI Ying-chun. Determinate Concentration of Methotrexate in Acute Lymphoblasticleukemia Children by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 108-110. DOI：

摘要

目的: 建立一快速、准确的RP-HPLC方法测定人血清中甲氨蝶呤的浓度。方法: 以阿魏酸内标

采用Agilent 1100系列高效液相色谱仪

以大连依利特Kromasil C18柱(4.6 mm×250 mm

5 μm)为分析柱;流动相乙腈-磷酸缓冲盐溶液(15 ∶85

pH 6.8);流速1.0 mL ·min-1;检测波长306 nm;柱温30 ℃。血浆样品用乙腈沉淀蛋白后

取上清液

在60 ℃下经氮气挥干

残渣用流动相定容

取20 μL进样分析。结果: 色谱峰分离良好

无干扰。线性方程为Y=-1.65×10-2X+0.355

r=0.999 5

高、中、低3个浓度的样品萃取回收率均大于75%。结论: 该方法是一种可靠、快速灵敏的检测方法

适用于甲氨蝶呤的血药浓度监测。

Abstract

Objective: To develop a HPLC method to determine the concentration of methotrexatate (MTX) in human plasma. Method: The ferulic acid was used as inter standard (I.S.) The analytical column was a Kromasil C18 column connected with the Agilent 1100 HPLC system. The isocratic mobile phase was the acetonitrile and buffer of phosphate acid (pH 6.8) with the rate of 15 ∶85.The detective UV wavelength was 306 nm and the column temperature was 30 ℃. The plasma samples

added ferulic acid as internal standard

were deposited down the protein with acetonitrile and the upper phase was evaporated by nitrogen at 60 ℃. The remains was dissolved with mobile phase

20 μL was injected into the sampler. Result: The chromatography was good and not interfered by the components of the plasma. The linear equation was Y=-1.65×10-2X+0.355

r=0.999 5. Conclusion: This is a accurate

sensitive and conventient method for the dertermination of MTX in the therapeutic drug monitoring.

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