Study on Optimization of Extraction Technology for Total Alkaloids of Aconitum flavum by Orthogonal Design Method

DU Tao; CHEN Hong-gang; LIN Li; WEI Xue-ming

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Study on Optimization of Extraction Technology for Total Alkaloids of Aconitum flavum by Orthogonal Design Method

更新时间：2024-01-04

- Study on Optimization of Extraction Technology for Total Alkaloids of Aconitum flavum by Orthogonal Design Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 3, Pages: 20-22(2011)
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- Published：2011
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DU Tao, CHEN Hong-gang, LIN Li, et al. Study on Optimization of Extraction Technology for Total Alkaloids of Aconitum flavum by Orthogonal Design Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 20-22.
DOI：

DU Tao, CHEN Hong-gang, LIN Li, et al. Study on Optimization of Extraction Technology for Total Alkaloids of Aconitum flavum by Orthogonal Design Method[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 20-22. DOI：

摘要

目的 :优选铁棒锤生物碱的最佳提取工艺。方法 :选择溶剂、提取次数、洗涤次数为考察因素

以总生物碱的含量为考察指标

采用正交试验法优选铁棒锤生物碱的最佳提取工艺。结果: 铁棒锤生物碱的最佳提取工艺为浸提次数3次

提取溶剂为乙醚-三氯甲烷(3 ∶1)

洗涤1次。结论 :优选得到的提取工艺稳定性好、得率高、简便可行。

Abstract

Objective: To optimize the extraction technology for total alkaloids in the root of Aconitum flavum . Method: Dissolvent

times of extraction and washing were the test factors and the total alkaloids were the test index. The orthogonal design was used to optimize the extraction technology of total alkaloids. Result: The optimal extraction method was as follows: the ether:chloroform (3 ∶1) as dissolvent

extraction 3 times and washing once. Conclusion: The technology after optimization had a good stability

the yield of total alkaloids was high

and the method was easy to operate.

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Related Institution

Guiyang College of Traditional Chinese Midicine

Second Affiliated Hospital of Guiyang College of Traditional Chinese Midicine

College of Pharmacy, Chengdu University of TCM

Key Laboratory of Active Ingredients in Traditional Chinese Medicine(TCM) Co-constructed by Jilin Province and Ministry of Education, Changchun University of Chinese Medicine

Research and Development Centre of New Drugs,Guangxi University of Chinese Medicine

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