Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats

TANG Dan-li; LIU Zhai-hua; SUI Yu; TONG Lin; ZHANG Hua-min

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Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats

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- Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 14, Pages: 161-164(2010)
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- Published：2010
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TANG Dan-li, LIU Zhai-hua, SUI Yu, et al. Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 161-164.
DOI：

TANG Dan-li, LIU Zhai-hua, SUI Yu, et al. Comparative Studies on Effects of Three Different Chinese Medicine Treatments on NF-кB Signal Transduction Pathway of Myocardial Ischemia-reperfusion Injury in Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 161-164. DOI：

摘要

目的: 观察中医不同治法方药对大鼠心肌缺血再灌注损伤(I/R)核因子-кB(NF-кB)信号转导通路的影响。方法: 将60只SD大鼠随机分为5组:假手术组、模型组、祛痰宽胸组、活血化瘀组及痰瘀同治组。可逆性冠脉左前降支结扎缺血30 min再灌注2 h复制I/R模型

应用Western blotting 及RT-PCR法检测各组大鼠心肌核因子IкB诱导激酶(NIK)、IкB激酶β(IKKβ)、核转录因子抑制蛋白(IкBα)及NF-кBp65 mRNA的表达。结果: 与模型组比较

各治疗组均能降低心肌NIK

IKKβ

NF-кBp65 mRNA水平并上调IкBα蛋白表达(P<0.01)。结论: 祛痰宽胸法、活血化瘀法及痰瘀同治法均对I/R大鼠有保护作用

其主要作用机制可能是通过抑制NIK

IKKβ蛋白表达

减少IкBα蛋白的磷酸化降解而增加其蛋白水平

从而抑制NF-кB的过度活化

发挥其对缺血再灌注损伤心肌的保护作用。

Abstract

Objective: To investigate the influence of different Chinese medicine treatments on NF-кB signal transduction pathway of myocardial ischemia-reperfusion(I/R) injury in rats. Method: Sixty SD rats were divided into 5 groups randomly

sham-operated group: model group

removing phlegm and enlarging chest group

activating blood and dissolving stasis group

treating both phlegm and blood stasis group. The model of I/R of the myocardium was reproduced by ligation of left descending artery for 30 min followed by releasing the ligation for 2 hours in rats. NIK

IKKβ and IкBα protein expression of myocardial tissues in rats were detected by Western blotting method. And the levels of NF-кBp65 mRNA were determined with RT-PCR. Result: Compared with model group

the expression of NIK

IKKβ and NF-кBp65 mRNA were lower

and the protein expression of IкBα was increased in all groups treated by different Chinese medicine treatments(P<0.01). Conclusion: The removing phlegm and enlarging chest method

activating blood and dissolving stasis method

treating both phlegm and blood stasis method can protect myocardium from I/R. The mechanism of action was related to inhibiting the protein expression of NIK

IKKβ and enhancing the expression of IкBα

restraining the activation of NF-кB.

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Related Author

TANG Dan-li1

LIU Zhai-hua2

SUI Yu1

TONG Lin3

ZHANG Hua-min3

HU Longxiao

GAO Jiabei

MA Weihao

Related Institution

Institute of Information on Chinese Medicine,China Academy of Chinese Medical Sciences

Institute of Basic Theories of Chinese Medicine,China Academy of Chinese Medical Sciences

Experimental Research Center,China Academy of Chinese Medical Sciences

Natural History Museum of China

Beijing Key Laboratory of Chinese Materia Pharmacology，National Clinical Research Center of Traditional Chinese Medicine for Cardiovascular Diseases，Institute of Basic Medical Sciences， Xiyuan Hospital，China Academy of Chinese Medical Sciences

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