Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 6, Pages: 71-73(2011)
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Published:2011
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LIU Yuan, SONG Zhi-zhao, LI Wen-qi. HPLC Fingerprint Analysis of [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 71-73.
DOI:
LIU Yuan, SONG Zhi-zhao, LI Wen-qi. HPLC Fingerprint Analysis of [J]. Chinese journal of experimental traditional medical formulae, 2011, 17(6): 71-73.DOI:
Objective: To establish the fingerprint of leaves of Adinandra nitida by RP-HPLC. Method: The chromatographic conditions were as follows: a Kromasil C18 column was used; the mobile phase was composed of acetonitrile-0.1% phosphoric acid with gradient elution; the flow rate was 1 mL ·min-1 and the UV absorbance detection was set at 254 nm. Result: Under the selected chromatographic conditions
10 batches of HPLC fingerprint of A. nitida with good similarity were obtained
no less than 0.9. Conclusion: Quality of A. nitida can be controlled effectively by HPLC- UV fingerprint.