Objective: To explore the in vitro effect of the toxin of Selenocosmia huwena on inhibiting the growth and inducing the death of cells of neurogliocytoma

and activating caspase-3. Method: MTT was applied to obtain the growth curves of cell affected by different concentrations of toxin of S.huwena for 24 h and 48 h. AnnexinV-FITC was used to test the death rate. Absorption spectrometry was adopted to measure the relative activity of caspase-3. Result: The toxin of S.huwena evidently inhibited the growth of U251

and it could induce the death of cells neurogliocytoma with concentration-dependent manner. With cells affected by different concentrations of toxin for 24 h

we found that the activity of caspase-3 reached the maximum at 48 h with concentration-dependent manner. Conclusion: The toxin of S.huwena evidently inhibits the growth of U251 and induces the death of U251

and the process include the activation of caspase-3.