Objective: To establish a determination method for quality control of Rougan capsule. Method: The contents of salvianolic acid B

curcumin

demethoxycurcumin and bisdemethoxycurcumin in Rougan capsule were determined by HPLC-DAD. These compounds were analyzed with a Apollo C18 column (4.6 mm×250 mm

5 μm) by gradient elution using 4% acetic acid-acetonitrile as the mobile phase

the flow rata was 1.0 mL ·min-1

the column temperature was maintained at 30 ℃ and the wavelength was detected at 286 and 426 nm. The content of tauroursodeoxycholic acid in Rougan capsule was determined by HPLC-ELSD. It was analyzed with a Apollo C18 column (4.6 mm×250 mm

5 μm) by isocratic elution using acetonitrile-acetic acid-ammonium acetate solution (pH 4.5) as the mobile phase

the flow rate was 0.8 mL ·min-1

the temperature of the drift tube was set at 80℃

the pressure of nebulizing gas (air) was at 35 psi

the temperature of the atomizer was at 36 ℃. Result : All these compounds were linear in the range of 0.148 0-1.850

0.125 6-1.570

0.043 2-0.540 0

0.033 6-0.420 0 and 0.972 0-12.15 μg (r=0.999 3-0.999 9)

respectively. The RSDs of the precision

reproducibility and stability test were less than 3%; the average recovery of the method was in the range of 97.8%-101.7%. Conclusion : The established method is simple

accurate

and repeatable and can be used for quality control of Rougan capsule.