Objective: To study on the extraction and assaying of Huidouba polysaccharides

to provide new clues for drug treatment in clinical diabetes

and to develop and research new materials for treating diabetes mellitus

especially those from natural resources that is able to treat diabetes mellitus and no obvious side effects

which is of great significance. Method: The method of decocting with water and depositing with alcohol was adopted to extract polysaccharides from Huidouba. And after phenol-sulfuric acid reaction

the method of absorption spectrometry was used to determine the polysaccharides content of Huidouba. Result: The standard curve was linear in the range of 15-50 mg ·L-1 with correlation coefficient of r=0.999 3.The linear regression equation was A=0.015 2C+0.019 2.The average content of total sugars of Huidouba polysaccharides was 99.92% calculated using the conversion factor f=3.347 3. Conclusion: The method of phenol-sulfuric acid reaction used in determining the content of polysaccharides is simple in operation with high sensitivity

good stability and reproducibility

which can be used for quantity control of the polysaccharides content of Huidouba.