Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC

WEI Jian-hua; LI Yao-hua; LIANG Chen-yan; ZENG Wan-he; HUANG Jie-fang; ZHEN Han-shen

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Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC

更新时间：2024-01-04

- Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 17, Issue 3, Pages: 93-95(2011)
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- Published：2011
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WEI Jian-hua, LI Yao-hua, LIANG Chen-yan, et al. Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 93-95.
DOI：

WEI Jian-hua, LI Yao-hua, LIANG Chen-yan, et al. Determination of Protocatechuic Acid, Chlorgenic Acid and Caffeic Acid in Sangye Dispensing Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 93-95. DOI：

摘要

目的 :建立桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸的高效液相测定方法。方法 :采用Hypersil ODS2 C18(4.6 mm×250 mm

5 μm)色谱柱

以乙腈-0.1%磷酸为流动相

梯度洗脱

流速1.0 mL ·min-1

柱温为20 ℃

检测波长258

327 nm。结果 :原儿茶酸、绿原酸和咖啡酸分别在0.032 2~0.161 0μg

0.125 6~1.256μg和0.035 1~0.351 μg呈良好线性关系;平均回收率分别为102.1%、101.1%和101.5%。结论 :该法操作简便

分离效果理想

可用于测定桑叶配方颗粒中原儿茶酸、绿原酸和咖啡酸的测定。

Abstract

Objective: To establish the methods for determining protocatechuic acid

chlorgenic acid and caffeic acid in Sangye dispensing granules by HPLC. Method: The column of Hypersil ODS2 C18 column (4.6 mm× 250 mm

5 μm) was used. The mobile phase consisted of acetonitrile-0.1% phosphoric acid with gradient elution. The flow rate was 1.0 mL ·min-1. The column temperature was at 20 ℃

the detection wavelength was at 258 nm

328 nm. Result: The average recovery of protocatechuic acid

chlorgenic acid and caffeic acid was 102.1%

101.1% and 101.5% respectively. Protocatechuic acid

chlorgenic acid and caffeic acid were linear in the range of 0.032 2-0.161 0 μg

0.125 6-1.256 μg and 0.035 1-0.351 μg respectively. Conclusion: The method is simple

accurate and can used to determine the content of the protocatechuic acid in mulberry formula granules.

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Related Author

XING Jun-bo1

WANG Zhao-hong2

NING Kang-jian

FENG Hui-ping

HE Sheng-li

WU Huai-en

LAO Shen

WANG Wen-hui

Related Institution

Liaoning University of Traditional Chinese Medicine

Anhui Science and Technology University,Fengyang

Tianjin Pharmaceutical Group Xinzheng Co.,Ltd.,Xinzheng

Shanghai Kaibao Pharmaceutical Co.Ltd.

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

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