Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry

LIU Li-feng

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Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry

更新时间：2024-01-04

- Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 16, Pages: 62-63(2010)
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- Published：2010
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LIU Li-feng. Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 62-63.
DOI：

LIU Li-feng. Determination of Bilirubin in Nuihuang Zhibao Pills by Ultraviolet Spectrophotometry[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(16): 62-63. DOI：

摘要

目的 :建立牛黄至宝丸中胆红素的含量测定方法。方法: 本实验采用紫外分光光度法

以药典方法测定胆红素的含量

用重氮化试剂显色

对样品的提取检测波长为533 nm。结果: 牛黄至宝丸中胆红素的含量限度为不低于29.8 μg/丸

胆红素浓度在0.009 8~0.049 8 mg ·mL-1与吸收度呈良好的线性关系(r=0.999 9

n=5)

平均回收率为98.74%(RSD 0.45%

n=6)。结论: 该法简便、快速、稳定、可靠

可用于测定牛黄至宝丸中胆红素的含量。

Abstract

Objective: To establish a method of determination of bilirution in Niuhuang Zhibao Pills. Method: Bilirubin was determined by UV spectrophotometry according to Pharmacopoeia.The detection wavelength was at 533 nm. Result: The arerage levels of bilirubin in the Niuhuang Zhibao Pills was 29.8 μg one pill.Bilirubin was linear in the range of 0.009 8~0.049 8 mg ·mL-1with r=0.999 9

(n=5).The average recovery (n=6) was 98.74%(RSD 0.45%). Conclusion: The methods were proved to be simple sensitive and reproducible.It can be used for the qualitycontrol of Niuhuang Zhibao Pills.

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Guo-ming XIE

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Yan-ping ZHOU

Jin HAN

Ba-tu JIRIMU

SHI Ying2

JIN Xiang-qun1

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Related Institution

Academy of Mongolian Medicine,Inner Mongolia Medical University

Fifth Medical Center of People′s Liberation Army of China (PLA) General Hospital

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