Objective: To establish the method for determination of the content of linoleic acid and α-linoleic acid in hemp fruit by GC

and provide evidence for evaluating quality and criterion. Method: The GC separation was performed on a ZB-WAX column (0.5 μm×0.32 mm×30 m) at 190 ℃

inject temperature at 250 ℃

FID detecter temperature was at 250 ℃

gas as N2 (99.99%); flow rate was 1.0 mL ·min-1

air 450 mL ·min-1

H2 45 mL ·min-1. Result: The content of linoleic acid and α-linoleic acid in hemp fruit was 31.42%-37.21%

8.02%-9.65%. Conclusion: The method can be used to control the quality of linoleic acid and α-linoleic acid in hemp fruit

which is accurate

convenient and repeatable.