Determination of Content of Adenosine in Pinellia pedatisecta from Different Districts by HPLC
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Determination of Content of Adenosine in Pinellia pedatisecta from Different Districts by HPLC
Chinese Journal of Experimental Traditional Medical FormulaeVol. 17, Issue 3, Pages: 77-79(2011)
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Published:2011
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ZHOU Cheng-hui, LI Chun-xue, ZHANG Jia-xiao, et al. Determination of Content of Adenosine in Pinellia pedatisecta from Different Districts by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 77-79.
DOI:
ZHOU Cheng-hui, LI Chun-xue, ZHANG Jia-xiao, et al. Determination of Content of Adenosine in Pinellia pedatisecta from Different Districts by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2011, 17(3): 77-79.DOI:
Determination of Content of Adenosine in Pinellia pedatisecta from Different Districts by HPLC
Objective: To establish an HPLC method to determine the content of adenosine in Pinellia pedatisecta from different districts. Method: Experiment was carried out on Kromasil C18 (4.6mm×200mm
5μm) column with mobile phase consisted of methanol-water (15 ∶85) at a flow rate of 1.0 mL ·min-1.The detection wavelength was at 260 nm and the column temperature was at 25 ℃. Result: The linear range of adenosine was 9.84-78.72mg ·L-1 (r=0.999 7).The average recovery was 99.01%
RSD 1.94% (n=6). The adenosine content of P. pedatisecta from different districts ranged in order was as follows: Heze>Anguo>Hanzhong>Baoding>Bozhou>Liuyang. Conclusion: The method is quick
simple and reproducible for the determination of adenosine in P. pedatisecta.
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