Effects of Sanhuang Yishen Capsule on Expression of TGF-1 and VEGF in Kidney of Diabetic Rats
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Effects of Sanhuang Yishen Capsule on Expression of TGF-1 and VEGF in Kidney of Diabetic Rats
Chinese Journal of Experimental Traditional Medical FormulaeVol. 16, Issue 14, Pages: 143-146(2010)
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Published:2010
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CHI Xiu-e, WANG Yuan-song, TIAN Feng-sheng, et al. Effects of Sanhuang Yishen Capsule on Expression of TGF-1 and VEGF in Kidney of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 143-146.
DOI:
CHI Xiu-e, WANG Yuan-song, TIAN Feng-sheng, et al. Effects of Sanhuang Yishen Capsule on Expression of TGF-1 and VEGF in Kidney of Diabetic Rats[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(14): 143-146.DOI:
Effects of Sanhuang Yishen Capsule on Expression of TGF-1 and VEGF in Kidney of Diabetic Rats
Objective: To observe and verify the intervention action of Sanhuang Yishen Capsule in rats with early diabetic nephropathy
and to study its possible mechanism
so as to provide experimental evidence for the clinic prevention of diabetic nephropathy. Method: Diabetic rats(n=13/group) were randomly divided into diabetes model group
low-dose Sanhuang Yishen Capsule treated group(0.4 g ·kg-1 ·d-1)
middle-dose Sanhuang Yishen Capsule treated group(0.8 g ·kg-1 ·d-1)
high-dose Sanhuang Yishen Capsule treated group(1.6 g ·kg-1 ·d-1). Ten SD rats were selected as control group. At the end of the 8th week
we detected blood glucose
glycosylated hemoglobin
creatinine
urea nitrogen
triglyceride
cholesterol and kidney weight/body weight index. The expression of renal TGF-β1 and VEGF were assessed by immune-histochemistry. Result: After treatment of 8 weeks
compared with the diabetes model group the middle-dose and the high-dose Sanhuang Yishen Capsule groups showed obviously decreases in kidney weight/body weight index and the level of serum creatinine
urea nitrogen (P<0.01). The expression of TGF-β1 and VEGF in kidney of middle-
high-dose Sanhuang Yishen Capsule treated group rats was lower than that of the diabetes model group(P<0.05). Conclusion: Sanhuang Yishen Capsule can reduce the expression of TGF-β1 and VEGF in the kidney of diabetic rats and delay of renal injury in diabetic rats.