Determination of Content of Oligosaccharide and Catalpol in Rehmannia Root by HPLC

QIU Jian-guo; ZHANG Ru-xue; JIA Zheng-ping; LI Mao-xing; FAN Peng-cheng; ZHANG Quan-long; WEI Li-li

您当前的位置：

首页 >

文章列表页 >

Determination of Content of Oligosaccharide and Catalpol in Rehmannia Root by HPLC

更新时间：2024-01-04

- Determination of Content of Oligosaccharide and Catalpol in Rehmannia Root by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 16, Issue 17, Pages: 110-113(2010)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：
  CLC：
- Published：2010
- 稿件说明：
移动端阅览
QIU Jian-guo, ZHANG Ru-xue, JIA Zheng-ping, et al. Determination of Content of Oligosaccharide and Catalpol in Rehmannia Root by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 110-113.
DOI：

QIU Jian-guo, ZHANG Ru-xue, JIA Zheng-ping, et al. Determination of Content of Oligosaccharide and Catalpol in Rehmannia Root by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2010, 16(17): 110-113. DOI：

摘要

目的: 考察不同产地的生地黄中所含地黄寡糖和梓醇的含量。方法: 采用水为溶媒

超声提取糖类成分

流动相用乙腈-水(72 ∶28)

流速1.0 mL ·min-1

NH2色谱柱分离

示差折光检测器检测

高效液相色谱法测定;采用甲醇为溶媒

超声提取梓醇

流动相用甲醇-0.1%磷酸溶液(1 ∶99)

流速1.0 mL ·min-1

C18色谱柱分离

检测波长210 nm

高效液相色谱法测定。结果: 产地不同

生地黄中的地黄寡糖成分、含量不同

梓醇含量不同。结论: 本方法操作简便、准确、快速、干扰少

适用于生地黄中地黄寡糖和梓醇的含量测定。

Abstract

ObJective: To contrast ingredients of rehmannia dride rhizome with different disct. Method: Carbohydrate was extracted by supersound and water used for solvent

was separated by NH2 chromatographic column and acetonitrile-water(72 ∶28) for mobile phase at 1.0 mL ·min-1

was detected by refraction detector and was assayed by high-performance liquid chromatography. Catalpinoside was extracted by supersound and methanol used for solvent

was separated by C18 chromatographic column and methanol-0.1%phosphoric acid solution at 1.0 mL ·min-1

was detected at λ=210 nm and was assayed by high-performance liquid chromatography. Result :Carbohydrate ingredients of rehmannia dride rhizome and prepared rhizome of rehmannia are conformity

but content are different. Content of catalpinoside have striking difference. Conclusion: The method is good in the aspect of reproducibility and sensitivity.It can be applied to contrast for the ingredients of Rehmannia dride rhizome and prepared rhizome of rehmannia.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Herbal Textual Research on Bruceae Fructus in Famous Classical Formulas

Herbal Textual Reseach on Haliotidis Concha in Famous Classical Formulas

HPLC Fingerprint Analysis and Multi-component Determination of Substance Benchmark of Yiweitang

Determination of Catalpol Content in Radix Rehmanniae from Genuine Producing Area by Near Infrared Spectroscopy

Related Author

YANG Chengzi

ZHAN Zhilai

CHEN Yanning

CHEN Yichong

ZHANG Yiqin

ZHUANG Yixue

XU Shuiyu

YANG Chengzi

Related Institution

School of Pharmacy，Fujian University of Traditional Chinese Medicine（TCM）

National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

Fujian Provincial TCM Raw Material Quality Monitoring Technical Service Center

School of Pharmacy，Fujian University of Traditional Chinese Medicine

College of Pharmaceutical Sciences， Southwest University

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰