Objective: To establish the quality standard for Niaoganning Granule. Method: The TLC was applied to identify Herba Glechomae Longitubae and Herba Pteridis Nervosae in this prescription

and aesculetin content in Herba Violae was determined by HPLC. A column (4.6 mm×250 mm

5 μm) was used with the mobile phase of methanol and 0.5% phosphoric acid (22 ∶78) at the detection wavelength of 345 nm. Result: Glechomae Longitubae and Pteridis Nervosae could be identified by TLC. The aesculetin linear rang of 20.08-2 080 ng

and the average recoverywas 96.3%

RSD 1.9%

Conclusion: The above methods are simple

sensitive

accurate and reproducible

and can be used for the quality control of this granule.